Unknown

Dataset Information

0

Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion.


ABSTRACT: Skeletal muscle development requires the cell-cell fusion of differentiated myoblasts to form muscle fibers. The actin cytoskeleton is known to be the main driving force for myoblast fusion; however, how actin is organized to direct intercellular fusion remains unclear. Here we show that an actin- and dynamin-2-enriched protrusive structure, the invadosome, is required for the fusion process of myogenesis. Upon differentiation, myoblasts acquire the ability to form invadosomes through isoform switching of a critical invadosome scaffold protein, Tks5. Tks5 directly interacts with and recruits dynamin-2 to the invadosome and regulates its assembly around actin filaments to strengthen the stiffness of dynamin-actin bundles and invadosomes. These findings provide a mechanistic framework for the acquisition of myogenic fusion machinery during myogenesis and reveal a novel structural function for Tks5 and dynamin-2 in organizing actin filaments in the invadosome to drive membrane fusion.

SUBMITTER: Chuang MC 

PROVIDER: S-EPMC6504888 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tks5 and Dynamin-2 enhance actin bundle rigidity in invadosomes to promote myoblast fusion.

Chuang Mei-Chun MC   Lin Shan-Shan SS   Ohniwa Ryosuke L RL   Lee Gang-Hui GH   Su You-An YA   Chang Yu-Chen YC   Tang Ming-Jer MJ   Liu Ya-Wen YW  

The Journal of cell biology 20190320 5


Skeletal muscle development requires the cell-cell fusion of differentiated myoblasts to form muscle fibers. The actin cytoskeleton is known to be the main driving force for myoblast fusion; however, how actin is organized to direct intercellular fusion remains unclear. Here we show that an actin- and dynamin-2-enriched protrusive structure, the invadosome, is required for the fusion process of myogenesis. Upon differentiation, myoblasts acquire the ability to form invadosomes through isoform sw  ...[more]

Similar Datasets

| S-EPMC4198353 | biostudies-literature
| S-EPMC3857171 | biostudies-literature
| S-EPMC3542790 | biostudies-literature
| S-EPMC2736864 | biostudies-other
| S-EPMC3091497 | biostudies-literature
| S-EPMC2823627 | biostudies-literature
| S-EPMC4546610 | biostudies-literature
| S-EPMC3062424 | biostudies-literature
| S-EPMC4602036 | biostudies-literature
| S-EPMC117532 | biostudies-literature