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Fak-Mapk, Hippo and Wnt signalling pathway expression and regulation in distraction osteogenesis.


ABSTRACT: OBJECTIVES:To investigate the mechanism of mechanical stimulation in bone formation and regeneration during distraction osteogenesis. MATERIALS AND METHODS:In this study, microarray technology was used to investigate the time course of bone-related molecular changes in distraction osteogenesis in rats. Real-time PCR and Western-blot analyses were used to confirm the expression of genes identified in microarrays. Meanwhile, we used a lentivirus vector to inhibit Fak expression, in order to identify the osteogenic effect of Fak and Fak-Mapk pathway during distraction osteogenesis. RESULTS:Several components of the Wnt and Hippo pathways were found to be up- or down-regulated during distraction osteogenesis by microarray. Meanwhile, it was found that Fak, Src, Raf-1, Erk1, Jnk and p38-Mapk were up-regulated during gradual distraction, compared with consolidation. To further determine whether Fak-Mapk pathway played an important role in distraction osteogenesis, Fak was disrupted with a lentivirus vector. The expressions levels of p-Fak, p-Erk1/2, p-JNK and p-p38Mapk were decreased. Meanwhile, a poor early and late osteogenesis effect was found in the shRNA-Fak group. CONCLUSION:It was inferred that the mechanical stimulus induces increased expression of Fak and activates Fak-Mapk pathway, by activation of Erk, Jnk and p38-Mapk pathway, and that Fak at least, in part, plays an important role in maintaining osteogenic effect by activating Fak-Mapk pathway during distraction osteogenesis.

SUBMITTER: Song J 

PROVIDER: S-EPMC6528869 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Fak-Mapk, Hippo and Wnt signalling pathway expression and regulation in distraction osteogenesis.

Song Jian J   Ye Bin B   Liu Hanghang H   Bi Ruiye R   Zhang Nian N   Hu Jing J   Luo En E  

Cell proliferation 20180305 4


<h4>Objectives</h4>To investigate the mechanism of mechanical stimulation in bone formation and regeneration during distraction osteogenesis.<h4>Materials and methods</h4>In this study, microarray technology was used to investigate the time course of bone-related molecular changes in distraction osteogenesis in rats. Real-time PCR and Western-blot analyses were used to confirm the expression of genes identified in microarrays. Meanwhile, we used a lentivirus vector to inhibit Fak expression, in  ...[more]

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