Dataset Information

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34⁺ cells.

ABSTRACT:

Objectives

Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.

Materials and methods

Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydrogel to achieve a slow release rate. CD34⁺ cells were cultured within the SCF-loaded HGDN hydrogel for 14 days. The cell number, phenotype and functional capacity were investigated after culture.

Results

The HGDN hydrogels had desirable properties and encapsulated SCF kept being released for more than 6 days. SCF remained the native bioactivity, and the proliferation of HSCs within the SCF-loaded HGDN hydrogel was not affected, although the consumption of SCF was only a quarter in comparison with the conventional culture. Moreover, CD34⁺ cells harvested from the SCF-loaded HGDN hydrogels generated more multipotent colony-forming units (CFU-GEMM).

Conclusion

The data suggested that the SCF-loaded HGDN hydrogel could support ex vivo culture of HSCs, thus providing a cost-effective culture protocol for HSCs.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC6528907 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Publications

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34<sup>+</sup> cells.

Zhang Yuanhao Y Pan Xiuwei X Shi Zhen Z Cai Haibo H Gao Yun Y Zhang Weian W

Cell proliferation 20171115 2

<h4>Objectives</h4>Stem cell factor (SCF) is considered as a commonly indispensable cytokine for proliferation of haematopoietic stem cells (HSCs), which is used in large dosages during ex vivo culture. The work presented here aimed to reduce the consumption of SCF by sustained release but still support cells proliferation and maintain the multipotency of HSCs.<h4>Materials and methods</h4>Stem cell factor was physically encapsulated within a hyaluronic acid/gelatin double network (HGDN) hydroge ...[more]

PMID: 29143396

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Project description:Diabetes can impair wound closure, which can give rise to major clinical problems. Most treatments for wound repair in diabetes remain ineffective. This study aimed to investigate the influence on wound closure of treatments using expanded human cord blood CD34(+) cells (CB-CD34(+) cells), freshly isolated CB-CD34(+) cells and a cytokine cocktail. The test subjects were mice with streptozotocin-induced diabetes. Wounds treated with fresh CB-CD34(+) cells showed more rapid repair than mice given the PBS control. Injection of expanded CB-CD34(+) cells improved wound closure significantly, whereas the injection of the cytokine cocktail alone did not improve wound repair. The results also demonstrated a significant decrease in epithelial gaps and advanced re-epithelialization over the wound bed area after treatment with either expanded CB-CD34(+) cells or freshly isolated cells compared with the control. In addition, treatments with both CB-CD34(+) cells and the cytokine cocktail were shown to promote recruitment of CD31(+)-endothelial cells in the wounds. Both the CB-CD34(+) cell population and the cytokine treatments also enhanced the recruitment of CD68-positive cells in the early stages (day 3) of treatment compared with PBS control, although the degree of this enhancement was found to decline in the later stages (day 9). These results demonstrated that expanded CB-CD34(+) cells or freshly isolated CB-CD34(+) cells could accelerate wound repair by increasing the recruitment of macrophages and capillaries and the reepithelialization over the wound bed area. Our data suggest an effective role in wound closure for both ex vivo expanded CB-CD34(+) cells and freshly isolated cells, and these may serve as therapeutic options for wound treatment for diabetic patients. Wound closure acceleration by expanded CB-CD34(+) cells also breaks the insufficient quantity obstacle of stem cells per unit of cord blood and other stem cell sources, which indicates a broader potential for autologous transplantation.

Dataset Information

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34⁺ cells.

Objectives

Materials and methods

Results

Conclusion

Publications

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34<sup>+</sup> cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34+ cells.

Objectives

Materials and methods

Results

Conclusion

Publications

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34<sup>+</sup> cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Sustained release of stem cell factor in a double network hydrogel for ex vivo culture of cord blood-derived CD34⁺ cells.