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Deciphering bacterial epigenomes using modern sequencing technologies.

ABSTRACT: Prokaryotic DNA contains three types of methylation: N6-methyladenine, N4-methylcytosine and 5-methylcytosine. The lack of tools to analyse the frequency and distribution of methylated residues in bacterial genomes has prevented a full understanding of their functions. Now, advances in DNA sequencing technology, including single-molecule, real-time sequencing and nanopore-based sequencing, have provided new opportunities for systematic detection of all three forms of methylated DNA at a genome-wide scale and offer unprecedented opportunities for achieving a more complete understanding of bacterial epigenomes. Indeed, as the number of mapped bacterial methylomes approaches 2,000, increasing evidence supports roles for methylation in regulation of gene expression, virulence and pathogen-host interactions.

SUBMITTER: Beaulaurier J

PROVIDER: S-EPMC6555402 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Deciphering bacterial epigenomes using modern sequencing technologies.

Beaulaurier John J Schadt Eric E EE Fang Gang G

Nature reviews. Genetics 20190301 3

Prokaryotic DNA contains three types of methylation: N6-methyladenine, N4-methylcytosine and 5-methylcytosine. The lack of tools to analyse the frequency and distribution of methylated residues in bacterial genomes has prevented a full understanding of their functions. Now, advances in DNA sequencing technology, including single-molecule, real-time sequencing and nanopore-based sequencing, have provided new opportunities for systematic detection of all three forms of methylated DNA at a genome-w ...[more]

PMID: 30546107

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Deciphering bacterial epigenomes using modern sequencing technologies.

Publications

Deciphering bacterial epigenomes using modern sequencing technologies.

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