Clonal V?6+V?4+ T cells promote IL-17-mediated immunity against Staphylococcus aureus skin infection.

ABSTRACT: T cell cytokines contribute to immunity against Staphylococcus aureus, but the predominant T cell subsets involved are unclear. In an S. aureus skin infection mouse model, we found that the IL-17 response was mediated by ?? T cells, which trafficked from lymph nodes to the infected skin to induce neutrophil recruitment, proinflammatory cytokines IL-1?, IL-1?, and TNF, and host defense peptides. RNA-seq for TRG and TRD sequences in lymph nodes and skin revealed a single clonotypic expansion of the encoded complementarity-determining region 3 amino acid sequence, which could be generated by canonical nucleotide sequences of TRGV5 or TRGV6 and TRDV4 However, only TRGV6 and TRDV4 but not TRGV5 sequences expanded. Finally, V?6+ T cells were a predominant ?? T cell subset that produced IL-17A as well as IL-22, TNF, and IFN?, indicating a broad and substantial role for clonal V?6+V?4+ T cells in immunity against S. aureus skin infections.

SUBMITTER: Marchitto MC 

PROVIDER: S-EPMC6561199 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature