Identification and prioritization of macrolideresistance genes with hypothetical annotation inStreptococcus pneumoniae.

ABSTRACT: Macrolide resistant Streptococcus pneumoniae infections have limited treatment options. While some resistance mechanisms are well established, ample understanding is limited by incomplete genome annotation (hypothetical genes). Some hypothetical genes encode a domain of unknown function (DUF), a conserved protein domain with uncharacterized function. Here, we identify and confirm macrolide resistance genes. We further explore DUFs from macrolide resistance hypothetical genes to prioritize them for experimental characterization. We found gene similarities between two macrolide resistance gene signatures from untreated and either erythromycin- or spiramycin-treated resistant Streptococcus pneumoniae. We confirmed the association of these gene sets with macrolide resistance through comparison

SUBMITTER: Goad B 

PROVIDER: S-EPMC6563660 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature