Ontology highlight
ABSTRACT:
SUBMITTER: Melville D
PROVIDER: S-EPMC6589570 | biostudies-literature | 2019 Feb
REPOSITORIES: biostudies-literature
Melville David D Gorur Amita A Schekman Randy R
Molecular biology of the cell 20181128 3
COPII-coated vesicles are the primary mediators of ER-to-Golgi trafficking. Sar1, one of the five core COPII components, is a highly conserved small GTPase, which, upon GTP binding, recruits the other COPII proteins to the ER membrane. It has been hypothesized that the changes in the kinetics of SAR1 GTPase may allow for the secretion of large cargoes. Here we developed a cell-free assay to recapitulate COPII-dependent budding of large lipoprotein cargoes from the ER. We identified fatty-acid bi ...[more]