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Three new serine-protease autotransporters of Enterobacteriaceae (SPATEs) from extra-intestinal pathogenic Escherichia coli and combined role of SPATEs for cytotoxicity and colonization of the mouse kidney.


ABSTRACT: Serine protease autotransporters of Enterobacteriaceae (SPATEs) are secreted proteins that contribute to virulence and function as proteases, toxins, adhesins, and/or immunomodulators. An extra-intestinal pathogenic E. coli (ExPEC) O1:K1 strain, QT598, isolated from a turkey, was shown to contain vat, tsh, and three uncharacterized SPATE-encoding genes. Uncharacterized SPATEs: Sha (Serine-protease hemagglutinin autotransporter), TagB and TagC (tandem autotransporter genes B and C) were tested for activities including hemagglutination, autoaggregation, and cytotoxicity when expressed in E. coli K-12. Sha and TagB conferred autoaggregation and hemagglutination activities. TagB, TagC, and Sha all exhibited cytopathic effects on a bladder epithelial cell line. In QT598, tagB and tagC are tandemly encoded on a genomic island, and were present in 10% of UTI isolates and 4.7% of avian E. coli. Sha is encoded on a virulence plasmid and was present in 1% of UTI isolates and 20% of avian E. coli. To specifically examine the role of SPATEs for infection, the 5 SPATE genes were deleted from strain QT598 and tested for cytotoxicity. Loss of all five SPATEs abrogated the cytopathic effect on bladder epithelial cells, although derivatives producing any of the 5 SPATEs retained cytopathic activity. In mouse infections, sha gene-expression was up-regulated a mean of sixfold in the bladder compared to growth in vitro. Loss of either tagBC or sha did not reduce urinary tract colonization. Deletion of all 5 SPATEs, however, significantly reduced competitive colonization of the kidney supporting a cumulative role of SPATEs for QT598 in the mouse UTI model.

SUBMITTER: Habouria H 

PROVIDER: S-EPMC6592367 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Three new serine-protease autotransporters of <i>Enterobacteriaceae</i> (SPATEs) from extra-intestinal pathogenic <i>Escherichia coli</i> and combined role of SPATEs for cytotoxicity and colonization of the mouse kidney.

Habouria Hajer H   Pokharel Pravil P   Maris Segolène S   Garénaux Amélie A   Bessaiah Hicham H   Houle Sébastien S   Veyrier Frédéric J FJ   Guyomard-Rabenirina Stéphanie S   Talarmin Antoine A   Dozois Charles M CM  

Virulence 20191201 1


Serine protease autotransporters of <i>Enterobacteriaceae</i> (SPATEs) are secreted proteins that contribute to virulence and function as proteases, toxins, adhesins, and/or immunomodulators. An extra-intestinal pathogenic <i>E. coli</i> (ExPEC) O1:K1 strain, QT598, isolated from a turkey, was shown to contain <i>vat, tsh</i>, and three uncharacterized SPATE-encoding genes. Uncharacterized SPATEs: Sha (<i><u>S</u></i>erine-protease <i><u>h</u></i>emagglutinin <i><u>a</u></i>utotransporter), TagB  ...[more]

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