Unknown

Dataset Information

0

Ionisation bias undermines the use of matrix-assisted laser desorption/ionisation for estimating peptide deamidation: Synthetic peptide studies demonstrate electrospray ionisation gives more reliable response ratios.


ABSTRACT:

Rationale

Although mass spectrometry (MS) is routinely used to determine deamination in peptide mixtures, the effects of the choice of ionisation source have not yet been investigated. In particular, matrix-assisted laser desorption/ionisation (MALDI) has become a popular tool with which to measure levels of glutamine deamidation in ancient proteins. Here we use model synthetic peptides to rigorously compare MALDI and electrospray ionisation (ESI).

Methods

We used two synthetic peptides, with glutamine (Q) in one substituted for glutamic acid (E) in the other, to investigate the suitability of MALDI and ESI sources for the assessment of deamidation in peptides using MS. We also compared measurements of the same Q- and E-containing peptide mixtures using two different mass analysers (time-of-flight (TOF) and Fourier transform ion cyclotron resonance (FT-ICR)).

Results

When standard mixtures of the Q- and E-containing peptides were analysed using MALDI, under-representation of the E-containing peptide was observed. This observation was consistent between analyses carried out using either TOF or FT-ICR-MS. When the same mixtures were analysed using ESI FT-ICR-MS, no ionisation bias was observed.

Conclusions

MALDI may not be a suitable ionisation method for the determination of deamidation in peptide mixtures. However, ESI was successfully used to determine the ratio in known mixtures of Q- and E-containing peptides. These preliminary observations warrant further investigation into ionisation bias when measuring deamidation in other peptide sequences.

SUBMITTER: Simpson JP 

PROVIDER: S-EPMC6594239 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Ionisation bias undermines the use of matrix-assisted laser desorption/ionisation for estimating peptide deamidation: Synthetic peptide studies demonstrate electrospray ionisation gives more reliable response ratios.

Simpson Joanna P JP   Fascione Martin M   Bergström Ed E   Wilson Julie J   Collins Matthew J MJ   Penkman Kirsty E H KEH   Thomas-Oates Jane J  

Rapid communications in mass spectrometry : RCM 20190601 12


<h4>Rationale</h4>Although mass spectrometry (MS) is routinely used to determine deamination in peptide mixtures, the effects of the choice of ionisation source have not yet been investigated. In particular, matrix-assisted laser desorption/ionisation (MALDI) has become a popular tool with which to measure levels of glutamine deamidation in ancient proteins. Here we use model synthetic peptides to rigorously compare MALDI and electrospray ionisation (ESI).<h4>Methods</h4>We used two synthetic pe  ...[more]

Similar Datasets

| S-EPMC8837636 | biostudies-literature
| S-EPMC5314351 | biostudies-literature
| S-EPMC3176668 | biostudies-literature
| S-EPMC6276080 | biostudies-literature
2017-01-10 | MTBLS385 | MetaboLights
| S-EPMC3259225 | biostudies-literature
| S-EPMC10701858 | biostudies-literature
| S-EPMC6541643 | biostudies-literature
| S-EPMC5887808 | biostudies-other
| S-EPMC6485486 | biostudies-literature