A rapid smartphone-based lactate dehydrogenase test for neonatal diagnostics at the point of care.

ABSTRACT: There is a growing recognition of the importance of point-of-care tests (POCTs) for detecting critical neonatal illnesses to reduce the mortality rate in newborns, especially in low-income countries, which account for 98 percent of reported neonatal deaths. Lactate dehydrogenase (LDH) is a marker of cellular damage as a result of hypoxia-ischemia in affected organs. Here, we describe and test a POC LDH test direct from whole blood to provide early indication of serious illness in the neonate. The sample-in-result-out POC platform is specifically designed to meet the needs at resource-limited settings. Plasma is separated from whole blood on filter paper with dried-down reagents for colorimetric reaction, combined with software for analysis using a smartphone. The method was clinically tested in newborns in two different settings. In a clinical cohort of newborns of Stockholm (n?=?62) and Hanoi (n?=?26), the value of R using Pearson's correlation test was 0.91 (p?

SUBMITTER: Halvorsen CP 

PROVIDER: S-EPMC6595069 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature