Ontology highlight
ABSTRACT:
SUBMITTER: Shimada M
PROVIDER: S-EPMC6598686 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Shimada Miho M Chen Wei-Yi WY Nakadai Tomoyoshi T Onikubo Takashi T Guermah Mohamed M Rhodes Daniela D Roeder Robert G RG
Molecular cell 20190319 2
Linker histone H1 has been correlated with transcriptional inhibition, but the mechanistic basis of the inhibition and its reversal during gene activation has remained enigmatic. We report that H1-compacted chromatin, reconstituted in vitro, blocks transcription by abrogating core histone modifications by p300 but not activator and p300 binding. Transcription from H1-bound chromatin is elicited by the H1 chaperone NAP1, which is recruited in a gene-specific manner through direct interactions wit ...[more]