Unknown

Dataset Information

0

Single upconversion nanoparticle imaging at sub-10 W cm-2 irradiance.


ABSTRACT: Lanthanide-doped upconversion nanoparticles (UCNPs) are promising single-molecule probes given their non-blinking, photobleach-resistant luminescence upon infrared excitation. However, the weak luminescence of sub-50 nm UCNPs limits their single-particle detection to above 10 kWcm-2 that is impractical for live cell imaging. Here, we systematically characterize single-particle luminescence for UCNPs with various formulations over a 106 variation in incident power, down to 8 Wcm-2. A core-shell-shell (CSS) structure (NaYF4@NaYb1-xF4:Erx@NaYF4) is shown to be significantly brighter than the commonly used NaY0.78F4:Yb0.2Er0.02. At 8 Wcm-2, the 8% Er3+ CSS particles exhibit a 150-fold enhancement given their high sensitizer Yb3+ content and the presence of an inert shell to prevent energy migration to defects. Moreover, we reveal power-dependent luminescence enhancement from the inert shell, which explains the discrepancy in enhancement factors reported by ensemble and previous single-particle measurements. These brighter probes open the possibility of cellular and single-molecule tracking at low irradiance.

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6599589 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Single upconversion nanoparticle imaging at sub-10 W cm<sup>-2</sup> irradiance.

Liu Qian Q   Zhang Yunxiang Y   Peng Chunte Sam CS   Yang Tianshe T   Joubert Lydia-Marie LM   Chu Steven S  

Nature photonics 20180806 9


Lanthanide-doped upconversion nanoparticles (UCNPs) are promising single-molecule probes given their non-blinking, photobleach-resistant luminescence upon infrared excitation. However, the weak luminescence of sub-50 nm UCNPs limits their single-particle detection to above 10 kWcm<sup>-2</sup> that is impractical for live cell imaging. Here, we systematically characterize single-particle luminescence for UCNPs with various formulations over a 10<sup>6</sup> variation in incident power, down to 8  ...[more]

Similar Datasets

| S-EPMC8295256 | biostudies-literature
| S-EPMC4636449 | biostudies-literature
| S-EPMC3653952 | biostudies-literature
| S-EPMC5797087 | biostudies-other
| S-EPMC5707587 | biostudies-literature
| S-EPMC5424175 | biostudies-literature
| S-EPMC4317689 | biostudies-literature
| S-EPMC208760 | biostudies-literature
| S-EPMC6079005 | biostudies-literature
| S-EPMC5630134 | biostudies-literature