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Exploring Wells-Dawson Clusters Associated With the Small Ribosomal Subunit.


ABSTRACT: The polyoxometalate P2W18 O626- , the Wells-Dawson cluster, stabilized the ribosome sufficiently for the crystallographers to solve the phase problem and improve the structural resolution. In the following we characterize the interaction of the Wells-Dawson cluster with the ribosome small subunit. There are 14 different P2W18 O626- clusters interacting with the ribosome, and the types of interactions range from one simple residue interaction to complex association of multiple sites including backbone interactions with a Wells-Dawson cluster. Although well-documented that bridging oxygen atoms are the main basic sites on other polyoxometalate interaction with most proteins reported, the W=O groups are the main sites of the Wells-Dawson cluster interacting with the ribosome. Furthermore, the peptide chain backbone on the ribosome host constitutes the main sites that associate with the Wells-Dawson cluster. In this work we investigate the potential of one representative pair of closely-located Wells-Dawson clusters being a genuine Double Wells-Dawson cluster. We found that the Double Wells-Dawson structure on the ribosome is geometrically sound and in line with other Double Wells-Dawson clusters previously observed in the solid state and solution. This information suggests that the Double Wells-Dawson structure on the ribosome is real and contribute to characterization of this particular structure of the ribosome.

SUBMITTER: Crans DC 

PROVIDER: S-EPMC6624422 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Exploring Wells-Dawson Clusters Associated With the Small Ribosomal Subunit.

Crans Debbie C DC   Sánchez-Lombardo Irma I   McLauchlan Craig C CC  

Frontiers in chemistry 20190705


The polyoxometalate P<sub>2</sub>W<sub>18</sub> O 62 6 - , the Wells-Dawson cluster, stabilized the ribosome sufficiently for the crystallographers to solve the phase problem and improve the structural resolution. In the following we characterize the interaction of the Wells-Dawson cluster with the ribosome small subunit. There are 14 different P<sub>2</sub>W<sub>18</sub> O 62 6 - clusters interacting with the ribosome, and the types of interactions range from one simple residue interactio  ...[more]

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