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First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study.


ABSTRACT: The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should become accepted in the field and be shown to lead to reproducible results. This first, community-based harmonization study on XL-MS is based on the results of 32 groups participating worldwide. The aim of this paper is to summarize the status quo of XL-MS and to compare and evaluate existing cross-linking strategies. Our study therefore builds the framework for establishing best practice guidelines to conduct cross-linking experiments, perform data analysis, and define reporting formats with the ultimate goal of assisting scientists to generate accurate and reproducible XL-MS results.

SUBMITTER: Iacobucci C 

PROVIDER: S-EPMC6625963 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study.

Iacobucci Claudio C   Piotrowski Christine C   Aebersold Ruedi R   Amaral Bruno C BC   Andrews Philip P   Bernfur Katja K   Borchers Christoph C   Brodie Nicolas I NI   Bruce James E JE   Cao Yong Y   Chaignepain Stéphane S   Chavez Juan D JD   Claverol Stéphane S   Cox Jürgen J   Davis Trisha T   Degliesposti Gianluca G   Dong Meng-Qiu MQ   Edinger Nufar N   Emanuelsson Cecilia C   Gay Marina M   Götze Michael M   Gomes-Neto Francisco F   Gozzo Fabio C FC   Gutierrez Craig C   Haupt Caroline C   Heck Albert J R AJR   Herzog Franz F   Huang Lan L   Hoopmann Michael R MR   Kalisman Nir N   Klykov Oleg O   Kukačka Zdeněk Z   Liu Fan F   MacCoss Michael J MJ   Mechtler Karl K   Mesika Ravit R   Moritz Robert L RL   Nagaraj Nagarjuna N   Nesati Victor V   Neves-Ferreira Ana G C AGC   Ninnis Robert R   Novák Petr P   O'Reilly Francis J FJ   Pelzing Matthias M   Petrotchenko Evgeniy E   Piersimoni Lolita L   Plasencia Manolo M   Pukala Tara T   Rand Kasper D KD   Rappsilber Juri J   Reichmann Dana D   Sailer Carolin C   Sarnowski Chris P CP   Scheltema Richard A RA   Schmidt Carla C   Schriemer David C DC   Shi Yi Y   Skehel J Mark JM   Slavin Moriya M   Sobott Frank F   Solis-Mezarino Victor V   Stephanowitz Heike H   Stengel Florian F   Stieger Christian E CE   Trabjerg Esben E   Trnka Michael M   Vilaseca Marta M   Viner Rosa R   Xiang Yufei Y   Yilmaz Sule S   Zelter Alex A   Ziemianowicz Daniel D   Leitner Alexander A   Sinz Andrea A  

Analytical chemistry 20190522 11


The number of publications in the field of chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and to probe protein-protein interactions has increased during the last years. As the technique is now becoming routine for in vitro and in vivo applications in proteomics and structural biology there is a pressing need to define protocols as well as data analysis and reporting formats. Such consensus formats should becom  ...[more]

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