Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes.

ABSTRACT: Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.

SUBMITTER: Al'Khafaji AM 

PROVIDER: S-EPMC6661167 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature