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Lineage-specific expression of a T cell receptor variable gene promoter controlled by upstream sequences.

ABSTRACT: We have characterized the sequence contribution of DNA 5' of a functionally rearranged TCR promoter (V beta 8.1) on its T lineage-specific expression through the use of the chloramphenicol acetyl-transferase (CAT) reporter gene. A 230-bp fragment located 570 bp upstream of the determined transcription start site of the V beta 8.1 promoter confers a T lineage specificity of expression to a heterologous promoter. The inability of the V beta 8.1 promoter and its associated elements to function in B cells suggests the existence of a mechanism to prevent inappropriate V beta gene expression in B cells. Of considerable interest is the fact that both a B cell-specific and a nontissue-specific enhancer element were incapable of stimulating significant expression of this promoter in B cells. We discuss the implication of these results on the process of rearrangement of both Ig and TCR genes, and the differentiation of the lymphoid system.

SUBMITTER: Diamond DJ

PROVIDER: S-EPMC2189238 | biostudies-other | 1989 Apr

REPOSITORIES: biostudies-other

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Lineage-specific expression of a T cell receptor variable gene promoter controlled by upstream sequences.

Diamond D J DJ Nelson F B FB Reinherz E L EL

The Journal of experimental medicine 19890401 4

We have characterized the sequence contribution of DNA 5' of a functionally rearranged TCR promoter (V beta 8.1) on its T lineage-specific expression through the use of the chloramphenicol acetyl-transferase (CAT) reporter gene. A 230-bp fragment located 570 bp upstream of the determined transcription start site of the V beta 8.1 promoter confers a T lineage specificity of expression to a heterologous promoter. The inability of the V beta 8.1 promoter and its associated elements to function in B ...[more]

PMID: 2522500

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