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Aptamer-field-effect transistors overcome Debye length limitations for small-molecule sensing.

ABSTRACT: Detection of analytes by means of field-effect transistors bearing ligand-specific receptors is fundamentally limited by the shielding created by the electrical double layer (the "Debye length" limitation). We detected small molecules under physiological high-ionic strength conditions by modifying printed ultrathin metal-oxide field-effect transistor arrays with deoxyribonucleotide aptamers selected to bind their targets adaptively. Target-induced conformational changes of negatively charged aptamer phosphodiester backbones in close proximity to semiconductor channels gated conductance in physiological buffers, resulting in highly sensitive detection. Sensing of charged and electroneutral targets (serotonin, dopamine, glucose, and sphingosine-1-phosphate) was enabled by specifically isolated aptameric stem-loop receptors.

SUBMITTER: Nakatsuka N 

PROVIDER: S-EPMC6663484 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Aptamer-field-effect transistors overcome Debye length limitations for small-molecule sensing.

Nakatsuka Nako N   Yang Kyung-Ae KA   Abendroth John M JM   Cheung Kevin M KM   Xu Xiaobin X   Yang Hongyan H   Zhao Chuanzhen C   Zhu Bowen B   Rim You Seung YS   Yang Yang Y   Weiss Paul S PS   Stojanović Milan N MN   Andrews Anne M AM  

Science (New York, N.Y.) 20180906 6412

Detection of analytes by means of field-effect transistors bearing ligand-specific receptors is fundamentally limited by the shielding created by the electrical double layer (the "Debye length" limitation). We detected small molecules under physiological high-ionic strength conditions by modifying printed ultrathin metal-oxide field-effect transistor arrays with deoxyribonucleotide aptamers selected to bind their targets adaptively. Target-induced conformational changes of negatively charged apt  ...[more]

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