Dataset Information

Dysbiotic gut microbes may contribute to hypertension by limiting vitamin D production.

ABSTRACT:

Background

Accumulating studies have suggested that gut microbiota (GM) dysbiosis and vitamin D3 deficiency each play an important role during the progression of hypertension (HTN). However, few studies have characterized the underlying interaction between GM shift and vitamin D3 deficiency in HTN patients.

Hypothesis

This study aimed to evaluate the possible crosstalk between GM dysbiosis and vitamin D deficiency in the pathogenesis of HTN.

Methods

In a cohort of 34 HTN patients and 15 healthy controls, we analyzed the fecal microbiota products, GM composition, and the interaction between GM and vitamin D3.

Results

Vitamin D3 was significantly decreased in feces of HTN patients (P = .006, vs controls) and was correlated with altered GM, including decreased Shannon index (R² = 0.1296, P = .0111) and Pielou evenness (R² = 0.1509, P = .0058). Moreover, vitamin D3 positively correlated with HTN-reduced bacterial genera, including Subdoligranulum (R² = 0.181, P = .0023), Ruminiclostridium (R² = 0.1217, P = .014), Intestinimonas (R² = 0.2036, P = .0011), Pseudoflavonifractor (R² = 0.1014, P = .0257), Paenibacillus (R² = 0.089, P = .0373), and Marvinbryantia (R² = 0.08173, P = .0464). Partial least squares structural equation modeling showed that 27.7% of the total effect of gut microbiome on HTN was mediated by limiting vitamin D production. Finally, receiver operating characteristic curve analysis revealed the predictive capacity of differential gut microbiome signatures and decreased vitamin D3 to distinguish HTN patients (AUC = 0.749, P = .006).

Conclusions

Our findings suggest that the GM dysbiosis contributing to the development of HTN might be partially mediated by vitamin D3 deficiency. Future studies involving the underlying mechanism and intervention strategies targeting microbiome composition and vitamin D3 to counteract the progression of HTN are warranted.

SUBMITTER: Zuo K

PROVIDER: S-EPMC6672427 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Publications

Dysbiotic gut microbes may contribute to hypertension by limiting vitamin D production.

Zuo Kun K Li Jing J Xu Qiuhua Q Hu Chaowei C Gao Yuanfeng Y Chen Mulei M Hu Roumu R Liu Ye Y Chi Hongjie H Yin Qing Q Cao Yudan Y Wang Pan P Qin Yanwen Y Liu Xiaoyan X Zhong Jiuchang J Cai Jun J Li Kuibao K Yang Xinchun X

Clinical cardiology 20190528 8

<h4>Background</h4>Accumulating studies have suggested that gut microbiota (GM) dysbiosis and vitamin D3 deficiency each play an important role during the progression of hypertension (HTN). However, few studies have characterized the underlying interaction between GM shift and vitamin D3 deficiency in HTN patients.<h4>Hypothesis</h4>This study aimed to evaluate the possible crosstalk between GM dysbiosis and vitamin D deficiency in the pathogenesis of HTN.<h4>Methods</h4>In a cohort of 34 HTN pa ...[more]

PMID: 31099039

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Project description:BACKGROUND:Solid organ transplant recipients show heterogeneity in the occurrence and timing of acute rejection episodes. Understanding the factors responsible for such variability in patient outcomes may lead to improved diagnostic and therapeutic approaches. Rejection kinetics of transplanted organs mainly depends on the extent of genetic disparities between donor and recipient, but a role for environmental factors is emerging. We have recently shown that major alterations of the microbiota following broad-spectrum antibiotics, or use of germ-free animals, promoted longer skin graft survival in mice. Here, we tested whether spontaneous differences in microbial colonization between genetically similar individuals can contribute to variability in graft rejection kinetics. RESULTS:We compared rejection kinetics of minor mismatched skin grafts in C57BL/6 mice from Jackson Laboratory (Jax) and Taconic Farms (Tac), genetically similar animals colonized by different commensal microbes. Female Tac mice rejected skin grafts from vendor-matched males more quickly than Jax mice. We observed prolonged graft survival in Tac mice when they were exposed to Jax mice microbiome through co-housing or fecal microbiota transplantation (FMT) by gastric gavage. In contrast, exposure to Tac mice did not change graft rejection kinetics in Jax mice, suggesting a dominant suppressive effect of Jax microbiota. High-throughput sequencing of 16S rRNA gene amplicons from Jax and Tac mice fecal samples confirmed a convergence of microbiota composition after cohousing or fecal transfer. Our analysis of amplicon data associated members of a single bacterial genus, Alistipes, with prolonged graft survival. Consistent with this finding, members of the genus Alistipes were absent in a separate Tac cohort, in which fecal transfer from Jax mice failed to prolong graft survival. CONCLUSIONS:These results demonstrate that differences in resident microbiome in healthy individuals may translate into distinct kinetics of graft rejection, and contribute to interpersonal variability in graft outcomes. The association between Alistipes and prolonged skin graft survival in mice suggests that members of this genus might affect host physiology, including at sites distal to the gastrointestinal tract. Overall, these findings allude to a potential therapeutic role for specific gut microbes to promote graft survival through the administration of probiotics, or FMT.

Dataset Information

Dysbiotic gut microbes may contribute to hypertension by limiting vitamin D production.

Background

Hypothesis

Methods

Results

Conclusions

Publications

Dysbiotic gut microbes may contribute to hypertension by limiting vitamin D production.

Similar Datasets

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