Ontology highlight
ABSTRACT:
SUBMITTER: Chen S
PROVIDER: S-EPMC6693498 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Chen Sean S Sun Sabrina S Moonen Dewi D Lee Clancy C Lee Angus Yiu-Fai AY Schaffer David V DV He Lin L
Cell reports 20190601 13
Genetically engineered mouse models harboring large sequence insertions or modifications are critical for a wide range of applications including endogenous gene tagging, conditional knockout, site-specific transgene insertion, and gene replacement; however, existing methods to generate such animals remain laborious and costly. To address this, we developed an approach called CRISPR-READI (CRISPR RNP electroporation and AAV donor infection), combining adeno-associated virus (AAV)-mediated HDR don ...[more]