A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations.

ABSTRACT:

Background

In most microbial cultivations D-glucose is the main carbon and energy source. However, quantification of D-glucose especially in small scale is still challenging. Therefore, we developed a FRET-based glucose biosensor, which can be applied in microbioreactor-based cultivations. This sensor consists of a glucose binding protein sandwiched between two fluorescent proteins, constituting a FRET pair. Upon D-glucose binding the sensor undergoes a conformational change which is translated into a FRET-ratio change.

Results

The selected sensor shows an apparent K_d below 1.5 mM D-glucose and a very high sensitivity of up to 70% FRET-ratio change between the unbound and the glucose-saturated state. The soluble sensor was successfully applied online to monitor th

SUBMITTER: Otten J 

PROVIDER: S-EPMC6704555 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature