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A flow-through microfluidic system for the detection of circulating melanoma cells.

ABSTRACT: We report the fabrication of polyaniline nanofiber (PANI)-modified screen-printed electrode (PANI/SPE) incorporated in a poly-dimethylsiloxane (PDMS) microfluidic channel for the detection of circulating tumor cells. We employed this device to detect melanoma skin cancer cells through specific immunogenic binding of cell surface biomarker melanocortin 1 receptor (MC1R) to anti-MC1R antibody. The antibody-functionalized PANI/SPE was used in batch-continuous flow-through fashion. An aqueous cell suspension of ferri/ferrocyanide at a flow rate of 1.5?mL/min was passed over the immunosensor, which allowed for continuous electrochemical measurements. The sensor performed exceptionally well affording an ultralow limit of quantification of 1 melanoma cell/mL, both in buffer and when mixed with peripheral blood mononuclear cells, and the response was log-linear over the range of 10-9000 melanoma cells/10?mL.

SUBMITTER: Anu Prathap MU

PROVIDER: S-EPMC6718307 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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A flow-through microfluidic system for the detection of circulating melanoma cells.

Anu Prathap Mylamparambil Udayan MU Castro-Pérez Edgardo E Jiménez-Torres José A JA Setaluri Vijaysaradhi V Gunasekaran Sundaram S

Biosensors & bioelectronics 20190717

We report the fabrication of polyaniline nanofiber (PANI)-modified screen-printed electrode (PANI/SPE) incorporated in a poly-dimethylsiloxane (PDMS) microfluidic channel for the detection of circulating tumor cells. We employed this device to detect melanoma skin cancer cells through specific immunogenic binding of cell surface biomarker melanocortin 1 receptor (MC1R) to anti-MC1R antibody. The antibody-functionalized PANI/SPE was used in batch-continuous flow-through fashion. An aqueous cell s ...[more]

PMID: 31336226

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