Unknown

Dataset Information

0

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter.

ABSTRACT: Analyses of circulating tumor cells have been shown to be effective for the detection of cancer relapse and prognosis prediction. However, research regarding its utility in sarcoma remains scarce. In this study, the microfluidic chip-type cell sorter On-chip Sort was used to construct a system for detecting circulating sarcoma cells (CSCs). A pilot study using normal fibroblast or sarcoma cell lines was designed to establish a reliable protocol to separate CSCs by On-chip Sort. A single CSC was separated and recovered from 10?ml of whole blood from a patient with locally advanced myxofibrosarcoma. The nonsynonymous mutation for KMT2B p.Ile2602Val identified in the formalin-fixed paraffin-embedded tumor sample was also confirmed in the CSC. Use of the developed protocol may allow CSCs to become an early predictor for metastasis and recurrence of sarcoma. Further, it may aid in optimizing post-operative therapies for patients without metastasis.

SUBMITTER: Hasegawa N 

PROVIDER: S-EPMC6934608 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Analyses of circulating tumor cells have been shown to be effective for the detection of cancer relapse and prognosis prediction. However, research regarding its utility in sarcoma remains scarce. In this study, the microfluidic chip-type cell sorter On-chip Sort was used to construct a system for detecting circulating sarcoma cells (CSCs). A pilot study using normal fibroblast or sarcoma cell lines was designed to establish a reliable protocol to separate CSCs by On-chip Sort. A single CSC was  ...[more]

Similar Datasets

Unknown Isolation and molecular analysis of circulating tumor cells from lung cancer patients using a microfluidic chip type cell sorter.
| S-EPMC6113429 | biostudies-literature
Unknown Microfluidic cell sorter (μFCS) for on-chip capture and analysis of single cells.
| S-EPMC3508764 | biostudies-literature
Unknown Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip.
| S-EPMC5506378 | biostudies-literature
Unknown Entrapment of Prostate Cancer Circulating Tumor Cells with a Sequential Size-Based Microfluidic Chip.
| S-EPMC6830444 | biostudies-literature
Unknown Universal marker and detection tool for human sarcoma circulating tumor cells.
| S-EPMC3959622 | biostudies-literature
Unknown Size-based detection of sarcoma circulating tumor cells and cell clusters.
| S-EPMC5668012 | biostudies-literature
Unknown An integrated microfluidic chip system for single-cell secretion profiling of rare circulating tumor cells.
| S-EPMC4266859 | biostudies-literature
Unknown Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip.
| S-EPMC6556143 | biostudies-literature
Unknown Detection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells.
| S-EPMC5546157 | biostudies-literature
Unknown Detection of circulating tumor cells in liquid biopsy from Ewing sarcoma patients.
| S-EPMC5765973 | biostudies-literature