Unknown

Dataset Information

0

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets.


ABSTRACT: A highly sensitive and selective fluorogenic sensing of L-Cysteine (L-Cys) was implemented based on gelatin stabilized gold nanoparticles decorated reduced graphene oxide (rGO/Au) nanohybrid. The rGO/Au nanohybrid was prepared by the one-pot hydrothermal method and well characterized by different physiochemical techniques. The nanohybrid exhibits a weak fluorescence of rGO due to the energy transfer from the rGO to Au NPs. The rGO/Au nanohybrid shows enhanced fluorescence activity due to the restoration of quenched fluorescence of rGO/Au nanohybrid in presence of L-Cys. The rGO/Au nanohybrid exhibits much lower detection limit of 0.51?nM for L-Cys with higher selectivity. The fluorescence sensing mechanism arose from the fluorescence recovery due to the stronger interaction between Au NPs and L-Cys, and consequently, the energy transfer was prevented between rGO and Au NPs. The practicability of rGO/Au sensor was implemented by invitro bioimaging measurements in Colo-205 (colorectal adenocarcinoma) and MKN-45 (gastric carcinoma) cancer live cells with excellent biocompatibility.

SUBMITTER: Thirumalraj B 

PROVIDER: S-EPMC7127153 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets.

Thirumalraj Balamurugan B   Dhenadhayalan Namasivayam N   Chen Shen-Ming SM   Liu Yan-Jin YJ   Chen Tse-Wei TW   Liang Po-Huang PH   Lin King-Chuen KC  

Sensors and actuators. B, Chemical 20171213


A highly sensitive and selective fluorogenic sensing of L-Cysteine (L-Cys) was implemented based on gelatin stabilized gold nanoparticles decorated reduced graphene oxide (rGO/Au) nanohybrid. The rGO/Au nanohybrid was prepared by the one-pot hydrothermal method and well characterized by different physiochemical techniques. The nanohybrid exhibits a weak fluorescence of rGO due to the energy transfer from the rGO to Au NPs. The rGO/Au nanohybrid shows enhanced fluorescence activity due to the res  ...[more]

Similar Datasets

| S-EPMC7477225 | biostudies-literature
| S-EPMC6719798 | biostudies-literature
| S-EPMC9140755 | biostudies-literature
| S-EPMC10214923 | biostudies-literature
| S-EPMC8301884 | biostudies-literature
| S-EPMC9043533 | biostudies-literature
| S-EPMC4539546 | biostudies-other
| S-EPMC6682028 | biostudies-literature
| S-EPMC6832145 | biostudies-literature
| S-EPMC7022660 | biostudies-literature