Ontology highlight
ABSTRACT:
SUBMITTER: Jia N
PROVIDER: S-EPMC6731140 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Jia Ning N Jones Roger R Sukenick George G Patel Dinshaw J DJ
Molecular cell 20190717 5
Target RNA binding to crRNA-bound type III-A CRISPR-Cas multi-subunit Csm surveillance complexes activates cyclic-oligoadenylate (cA<sub>n</sub>) formation from ATP subunits positioned within the composite pair of Palm domain pockets of the Csm1 subunit. The generated cA<sub>n</sub> second messenger in turn targets the CARF domain of trans-acting RNase Csm6, triggering its HEPN domain-based RNase activity. We have undertaken cryo-EM studies on multi-subunit Thermococcus onnurineus Csm effector t ...[more]