Recognition of Class II MHC Peptide Ligands That Contain ?-Amino Acids.

ABSTRACT: Proteins are composed of ?-amino acid residues. This consistency in backbone structure likely serves an important role in the display of an enormous diversity of peptides by class II MHC (MHC-II) products, which make contacts with main chain atoms of their peptide cargo. Peptides that contain residues with an extra carbon in the backbone (derived from ?-amino acids) have biological properties that differ starkly from those of their conventional counterparts. How changes in the structure of the peptide backbone affect the loading of peptides onto MHC-II or recognition of the resulting complexes by TCRs has not been widely explored. We prepared a library of analogues of MHC-II-binding peptides derived from OVA, in which at least one ?-amino acid residue was replaced with a homologous ?-amino acid residue. The latter contain an extra methylene unit in the peptide backbone but retain the original side chain. We show that several of these ?/?-peptides retain the ability to bind tightly to MHC-II, activate TCR signaling, and induce responses from T cells in mice. One ?/?-peptide exhibited enhanced stability in the presence of an endosomal protease relative to the index peptide. Conjugation of this backbone-modified peptide to a camelid single-domain Ab fragment specific for MHC-II enhanced its biological activity. Our results suggest that backbone modification offers a method to modulate MHC binding and selectivity, T cell stimulatory capacity, and susceptibility to processing by proteases such as those found within endosomes where Ag processing occurs.

SUBMITTER: Cheloha RW 

PROVIDER: S-EPMC6736755 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature