Unknown

Dataset Information

0

Generation of a protective murine monoclonal antibody against the stem of influenza hemagglutinins from group 1 viruses and identification of resistance mutations against it.


ABSTRACT: Vaccines that elicit broadly cross-neutralizing antibodies, including antibodies that target the conserved stem of hemagglutinin (HA), are being developed as a strategy for next-generation influenza vaccines that protect against influenza across multiple years. However, efficient induction of cross-neutralizing antibodies remains a challenge, and potential escape mutations have not been well characterized. Here we elicited cross-neutralizing antibodies by immunizing animals with the hemagglutinins from H5 and H9 subtype influenza A viruses that are sensitive to neutralization by stem antibodies. We further isolated and characterized an HA stem monoclonal antibody 4C2 that broadly neutralizes group 1 influenza viruses and identified HA mutations that reduced sensitivity to stem antibodies. Our results offer insights for next-generation influenza vaccine strategies for inducing cross-neutralizing antibodies.

SUBMITTER: Wang W 

PROVIDER: S-EPMC6742228 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications

Generation of a protective murine monoclonal antibody against the stem of influenza hemagglutinins from group 1 viruses and identification of resistance mutations against it.

Wang Wei W   Vassell Russell R   Song Hyo Sook HS   Chen Qiong Q   Keller Paul W PW   Verma Swati S   Alvarado-Facundo Esmeralda E   Wan Hongquan H   Schmeisser Falko F   Meseda Clement A CA   Weir Jerry P JP   Weiss Carol D CD  

PloS one 20190912 9


Vaccines that elicit broadly cross-neutralizing antibodies, including antibodies that target the conserved stem of hemagglutinin (HA), are being developed as a strategy for next-generation influenza vaccines that protect against influenza across multiple years. However, efficient induction of cross-neutralizing antibodies remains a challenge, and potential escape mutations have not been well characterized. Here we elicited cross-neutralizing antibodies by immunizing animals with the hemagglutini  ...[more]

Similar Datasets

| S-EPMC2829068 | biostudies-other
| S-EPMC5110155 | biostudies-literature
| S-EPMC2652660 | biostudies-literature
| S-EPMC5819343 | biostudies-literature
| S-EPMC7720065 | biostudies-literature
| S-EPMC8475512 | biostudies-literature
| S-EPMC7441100 | biostudies-literature
| S-EPMC4262278 | biostudies-literature
2017-03-31 | GSE96091 | GEO
| S-EPMC7020694 | biostudies-literature