Ontology highlight
ABSTRACT:
SUBMITTER: Wang X
PROVIDER: S-EPMC6753160 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Wang Xiaolin X Kirkpatrick John P JP Launay Hélène M M HMM de Simone Alfonso A Häussinger Daniel D Dobson Christopher M CM Vendruscolo Michele M Cabrita Lisa D LD Waudby Christopher A CA Christodoulou John J
Scientific reports 20190919 1
We describe an NMR approach based on the measurement of residual dipolar couplings (RDCs) to probe the structural and motional properties of the dynamic regions of the ribosome. Alignment of intact 70S ribosomes in filamentous bacteriophage enabled measurement of RDCs in the mobile C-terminal domain (CTD) of the stalk protein bL12. A structural refinement of this domain using the observed RDCs did not show large changes relative to the isolated protein in the absence of the ribosome, and we also ...[more]