Dataset Information

Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections.

ABSTRACT: Pigs are anatomically, genetically and physiologically comparable to humans and represent a natural host for influenza A virus (IAV) infections. Thus, pigs may represent a relevant biomedical model for human IAV infections. We set out to investigate the systemic as well as the local immune response in pigs upon two subsequent intranasal infections with IAV H1N1pdm09. We detected decreasing numbers of peripheral blood lymphocytes after the first infection. The simultaneous increase in the frequencies of proliferating cells correlated with an increase in infiltrating leukocytes in the lung. Enhanced perforin expression in ?? and ?? T cells in the respiratory tract indicated a cytotoxic T cell response restricted to the route of virus entry such as the nose, the lung and the bronchoalveolar lavage. Simultaneously, increasing frequencies of CD8?? expressing ?? T cells were observed rapidly after the first infection, which may have inhibited uncontrolled inflammation in the respiratory tract. Taking together, the results of this study demonstrate that experimental IAV infection in pigs mimics major characteristics of human seasonal IAV infections.

SUBMITTER: Schwaiger T

PROVIDER: S-EPMC6754157 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections.

Schwaiger Theresa T Sehl Julia J Karte Claudia C Schäfer Alexander A Hühr Jane J Mettenleiter Thomas C TC Schröder Charlotte C Köllner Bernd B Ulrich Reiner R Blohm Ulrike U

PloS one 20190920 9

Pigs are anatomically, genetically and physiologically comparable to humans and represent a natural host for influenza A virus (IAV) infections. Thus, pigs may represent a relevant biomedical model for human IAV infections. We set out to investigate the systemic as well as the local immune response in pigs upon two subsequent intranasal infections with IAV H1N1pdm09. We detected decreasing numbers of peripheral blood lymphocytes after the first infection. The simultaneous increase in the frequen ...[more]

PMID: 31539406

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Project description:ImportanceCardiovascular deaths and influenza epidemics peak during winter in temperate regions.ObjectivesTo quantify the temporal association between population increases in seasonal influenza infections and mortality due to cardiovascular causes and to test if influenza incidence indicators are predictive of cardiovascular mortality during the influenza season.Design, setting, and participantsTime-series analysis of vital statistics records and emergency department visits in New York City, among cardiovascular deaths that occurred during influenza seasons between January 1, 2006, and December 31, 2012. The 2009 novel influenza A(H1N1) pandemic period was excluded from temporal analyses.ExposuresEmergency department visits for influenza-like illness, grouped by age (?0 years and ?65 years) and scaled by laboratory surveillance data for viral types and subtypes, in the previous 28 days.Main outcomes and measuresMortality due to cardiovascular disease, ischemic heart disease, and myocardial infarction.ResultsAmong adults 65 years and older, who accounted for 83.0% (73?363 deaths) of nonpandemic cardiovascular mortality during influenza seasons, seasonal average influenza incidence was correlated year to year with excess cardiovascular mortality (Pearson correlation coefficients ?0.75, P???.05 for 4 different influenza indicators). In daily time-series analyses using 4 different influenza metrics, interquartile range increases in influenza incidence during the previous 21 days were associated with an increase between 2.3% (95% CI, 0.7%-3.9%) and 6.3% (95% CI, 3.7%-8.9%) for cardiovascular disease mortality and between 2.4% (95% CI, 1.1%-3.6%) and 6.9% (95% CI, 4.0%-9.9%) for ischemic heart disease mortality among adults 65 years and older. The associations were most acute and strongest for myocardial infarction mortality, with each interquartile range increase in influenza incidence during the previous 14 days associated with mortality increases between 5.8% (95% CI, 2.5%-9.1%) and 13.1% (95% CI, 5.3%-20.9%). Out-of-sample prediction of cardiovascular mortality among adults 65 years and older during the 2009-2010 influenza season yielded average estimates with 94.0% accuracy using 4 different influenza metrics.Conclusions and relevanceEmergency department visits for influenza-like illness were associated with and predictive of cardiovascular disease mortality. Retrospective estimation of influenza-attributable cardiovascular mortality burden combined with accurate and reliable influenza forecasts could predict the timing and burden of seasonal increases in cardiovascular mortality.

Dataset Information

Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections.

Publications

Experimental H1N1pdm09 infection in pigs mimics human seasonal influenza infections.

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