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Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1.

ABSTRACT: Stable cell lines can continuously produce a recombinant protein without the need to repeatedly engineer the genome. In a previous study HIPK1, Homeodomain-interacting Protein Kinase 1, was found to be a target of the microRNA miR-22 that, when repressed, improved expression of both an intracellular and a secreted protein. In this report, HEK293 cells stably over-expressing miR-22 were compared with HEK293 with knockout of HIPK1, executed by CRISPR/Cas9, for their ability to improve recombinant protein expression. In this model case of luciferase, over-expression of miR-22 improved overall activity 2.4-fold while the HIPK1 knockout improved overall activity 4.7-fold.

SUBMITTER: Inwood S

PROVIDER: S-EPMC6759407 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1.

Inwood Sarah S Abaandou Laura L Betenbaugh Michael M Shiloach Joseph J

New biotechnology 20190816

Stable cell lines can continuously produce a recombinant protein without the need to repeatedly engineer the genome. In a previous study HIPK1, Homeodomain-interacting Protein Kinase 1, was found to be a target of the microRNA miR-22 that, when repressed, improved expression of both an intracellular and a secreted protein. In this report, HEK293 cells stably over-expressing miR-22 were compared with HEK293 with knockout of HIPK1, executed by CRISPR/Cas9, for their ability to improve recombinant ...[more]

PMID: 31425885

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