Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display.

ABSTRACT: Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo-electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

SUBMITTER: Vragniau C 

PROVIDER: S-EPMC6763337 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature