Dataset Information

Complete Genome Sequences of Lytic Polysaccharide-Degrading Klebsiella pneumoniae Bacteriophages vB_KpnS_FZ10, vB_KpnP_FZ12, vB_KpnM_FZ14, and vB_KpnS_FZ41.

ABSTRACT: Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. At the same time, most nosocomial infections are caused by strains resistant to antibiotics. Here, we announce the complete genome sequences of four lytic polysaccharide-degrading bacteriophages, which will be used in complex therapeutic preparations.

SUBMITTER: Zurabov F

PROVIDER: S-EPMC6763647 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

Complete Genome Sequences of Lytic Polysaccharide-Degrading Klebsiella pneumoniae Bacteriophages vB_KpnS_FZ10, vB_KpnP_FZ12, vB_KpnM_FZ14, and vB_KpnS_FZ41.

Zurabov Fedor F Zhilenkov Evgeniy E

Microbiology resource announcements 20190926 39

Members of the genus <i>Klebsiella</i> are among the leading microbial pathogens associated with nosocomial infection. At the same time, most nosocomial infections are caused by strains resistant to antibiotics. Here, we announce the complete genome sequences of four lytic polysaccharide-degrading bacteriophages, which will be used in complex therapeutic preparations. ...[more]

PMID: 31558632

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Project description:BACKGROUND: Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies. METHODS: Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages. RESULTS AND CONCLUSIONS: Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against 254 clinical Enterobacteriaceae strains including multidrug resistant Klebsiella isolates producing extended-spectrum beta-lactamases (ESBLs). Based on their lytic potential, six of the phages were further characterised for burst size, physicochemical properties and sensitivity to restriction endonuclease digestion. In addition, five were fully sequenced. Multiple phage-encoded host resistance mechanisms were identified. The Siphoviridae phage genomes (KP16 and KP36) contained low numbers of host restriction sites similar to the strategy found in T7-like phages (KP32). In addition, phage KP36 encoded its own DNA adenine methyltransferase. The ?KMV-like KP34 phage was sensitive to all endonucleases used in this study. Dam methylation of KP34 DNA was detected although this was in the absence of an identifiable phage encoded methyltransferase. The Myoviridae phages KP15 and KP27 both carried Dam and Dcm methyltransferase genes and other anti-restriction mechanisms elucidated in previous studies. No other anti-restriction mechanisms were found, e.g. atypical nucleotides (hmC or glucosyl hmC), although Myoviridae phage KP27 encodes an unknown anti-restriction mechanism that needs further investigation.

Dataset Information

Complete Genome Sequences of Lytic Polysaccharide-Degrading Klebsiella pneumoniae Bacteriophages vB_KpnS_FZ10, vB_KpnP_FZ12, vB_KpnM_FZ14, and vB_KpnS_FZ41.

Publications

Complete Genome Sequences of Lytic Polysaccharide-Degrading Klebsiella pneumoniae Bacteriophages vB_KpnS_FZ10, vB_KpnP_FZ12, vB_KpnM_FZ14, and vB_KpnS_FZ41.

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