Ontology highlight
ABSTRACT:
SUBMITTER: Oller-Salvia B
PROVIDER: S-EPMC6771915 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Oller-Salvia Benjamí B Chin Jason W JW
Angewandte Chemie (International ed. in English) 20190704 32
Phage display is a powerful approach for evolving proteins and peptides with new functions, but the properties of the molecules that can be evolved are limited by the chemical diversity encoded. Herein, we report a system for incorporating non-canonical amino acids (ncAAs) into proteins displayed on phage using the pyrrolysyl-tRNA synthetase/tRNA pair. We improve the efficiency of ncAA incorporation using an evolved orthogonal ribosome (riboQ1), and encode a cyclopropene-containing ncAA (CypK) a ...[more]