Dataset Information

Molecular prevalence of Anaplasma phagocytophilum in sheep from Iraq.

ABSTRACT: Background:Tick-borne diseases are widely distributed among animal populations and are responsible for significant economic losses. However, little attention has been offered for screening such infections world widely. Anaplasma phagocytophilum is among those neglected tick-borne pathogens, particularly in the developing countries. Aim:This study was conducted to detect A. phagocytophilum infection among sheep in three governorates of Iraq (Babylon, Wasit, and Missan) and try to identify the potential tick vector responsible for A. phagocytophilum transmission among sheep in these analyzed regions. Methods:A total of 297 blood samples and 103 ticks were collected and examined for A. phagocytophilum by polymerase chain reaction using specific primers amplifying partial sequence for msp4 gene. Results:The results showed that about 14 out of 297 tested sheep were positive for A. phagocytophilum. There was no difference between A. phygocytophilum prevalence according to animal gender, age, and sampling period. Furthermore, our analysis showed that the main vectors of A. phagocytophilum: Ixodes scapularis, I. pacificus, or I. ricinus were not identified in three regions of Iraq (Rhipicephalus turanicus, Hyalomma anatolicum, and Hyalomma turanicum were identified). Conclusion:These results highlight the importance of the survey of the tick-borne bacterial infections in Iraq and in the Middle East region in general.

SUBMITTER: Hamzah KJ

PROVIDER: S-EPMC6794391 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Molecular prevalence of <i>Anaplasma phagocytophilum</i> in sheep from Iraq.

Hamzah Karrar Jasim KJ Hasso Saleem Amin SA

Open veterinary journal 20190825 3

<h4>Background</h4>Tick-borne diseases are widely distributed among animal populations and are responsible for significant economic losses. However, little attention has been offered for screening such infections world widely. <i>Anaplasma phagocytophilum</i> is among those neglected tick-borne pathogens, particularly in the developing countries.<h4>Aim</h4>This study was conducted to detect <i>A. phagocytophilum</i> infection among sheep in three governorates of Iraq (Babylon, Wasit, and Missan ...[more]

PMID: 31998617

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Project description:BackgroundAnaplasma phagocytophilum is wide spread throughout the world and impacts both human and animal health. Several distinct ecological clusters and ecotypes of the agent have been established on the basis of various genetic loci. However, information on the genetic variability of A. phagocytophilum isolates in China represents a gap in knowledge. The objective of this study was to determine the prevalence and genetic characterization of A. phagocytophilum in small ruminants in central and southeastern China.MethodsThe presence of A. phagocytophilum was determined in 421 blood samples collected from small ruminants by PCR. Positive samples were genetically characterized based on 16S rRNA and groEL genes. Statistical analyses were conducted to identify ecotypes of A. phagocytophilum strains, to assess their host range and zoonotic potential.ResultsOut of 421 sampled small ruminants, 106 (25.2%) were positive for A. phagocytophilum. The positive rate was higher in sheep (35.1%, 40/114) than in goats (26.4%, 66/307) (P < 0.05). Sequence analyses revealed that the isolates identified in this study were placed on two separate clades, indicating that two 16S rRNA variants of A. phagocytophilum were circulating in small ruminants in China. However, analysis using obtained groEL sequences in this study formed one cluster, which was separate from other known ecotypes reported in Europe. In addition, a novel Anaplasma sp. was identified and closely related to an isolate previously reported in Hyalomma asiaticum, which clustered independently from all recognized Anaplasma species.ConclusionsA molecular survey of A. phagocytophilum was conducted in sheep and goats from ten provinces in central and southeastern China. Two 16S rRNA variants and a new ecotype of A. phagocytophilum were identified in small ruminants in China. Moreover, a potential novel Anaplasma species was reported in goats. Our findings provide additional information on the complexity of A. phagocytophilum in terms of genetic diversity in China.

Dataset Information

Molecular prevalence of Anaplasma phagocytophilum in sheep from Iraq.

Publications

Molecular prevalence of <i>Anaplasma phagocytophilum</i> in sheep from Iraq.

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