Molecular evidence for Anaplasma phagocytophilum in Israel.
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ABSTRACT:
SUBMITTER: Keysary A
PROVIDER: S-EPMC2857299 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Obligate intracellular bacteria belonging to the genus Anaplasma spp. are responsible for causing a hemolytic disease called anaplasmosis in animals, as well as in humans. This study was aimed at the molecular identification and genetic analysis of responsible causative agents of anaplasmosis beyond those already reported. A survey was performed during July and August 2018 in the Jhang District, Punjab, Pakistan. Four hundred and fifty blood samples from asymptomatic, tick-infested cattle were collected on FTA cards and tested for the Anaplasma spp. presence using nested-polymerase chain reaction (PCR) methods. The 16S ribosomal RNA gene sequences generated from the positive samples were used for genetic analysis of Anaplasma spp. The nested-PCR results showed the presence of two Anaplasma spp. with an overall prevalence rate of 10.44%, where the prevalence of A. bovis and A. phagocytophilum was 7.78% and 2.66%, respectively. The study portrayed new molecular data on the prevalence of Anaplasma spp. in the studied cattle population, indicating a potential threat to the human population as well.
| S-EPMC6789598 | biostudies-literature
Project description:BACKGROUND: Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium. Currently, the whole range of animal species reservoirs for A. phagocytophilum in natural environment is still unknown. Therefore, the aim of this study was to estimate the prevalence of infection with A. phagocytophilum in the wild boar population in southern Belgium. RESULTS: In the frame of a targeted surveillance program, 513 wild boars were sampled during the hunting season 2011. A nested 16S rRNA PCR was used to screen the presence of A. phagocytophilum DNA in spleen of boars. Within 513 samples, 5 (0,97%) were tested PCR positive and identification was confirmed by sequencing. CONCLUSIONS: This study gives the first insight of presence of A. phagocytophilum in wild boars in southern Belgium.
| S-EPMC3976503 | biostudies-literature
Project description:Human granulocytic Anaplasmosis (HGA), is a tick-borne infectious disease transmitted by ticks, resulting in acute feverish episodes. The etiological agent is the bacteria Anaplasma phagocytophilum; which is spread by ticks of the genus Ixodes spp. to complete its life cycle. In Mexico, there is only one case report. The primary challenge is understanding how other bacteria affect or overlap with the clinical manifestation of the disease. Sample collection occurred over the period September 2017 through October 2019. Blood samples from human subjects were obtained immediately after they signed consent forms. We analyzed for the presence for A. phagocytophilum by serological (IFA IgG two times) and PCR targeting 16SrRNA and groEL genes, followed by DNA sequencing. All patients with a history of travel abroad were dismissed for this project. In total, 1924 patients participated and of these, 1014 samples across the country were analyzed. Of these, 85 (8.38%) had IFA results that ranged from 1:384 to 1:896. Of the positive samples, 7.10% were used for PCR. Significant clinical manifestations included: dizziness, nausea, petechial, epistaxis, enlarged liver and/or spleen and thrombocytopenia. Hospitalization of at least 1.5 days was necessary for 3.2% of patients. None of the cases analyzed were lethal. This is the first clinical manifestations along with serological test results and molecular analysis confirmed the presence of A. phagocytophilum resulting in HGA in patients from Mexico. Health institutions and medical practitioners in general should include diagnostic testing for HGA among high risk populations and should recognize it as a vector-borne emerging infectious disease in Mexico.
| S-EPMC8161817 | biostudies-literature
Project description:BackgroundNonhuman primates (NHPs) play a significant role in zoonotic spill-overs, serving as either reservoirs, or amplifiers, of multiple neglected tropical diseases, including tick-borne infections. Anaplasma phagocytophilum are obligate intracellular bacteria of the family Anaplasmatacae, transmitted by Ixodid ticks and cause granulocytic anaplasmosis (formerly known as Human Granulocytic Ehrlichiosis (HGE)) in a wide range of wild and domestic mammals and humans too. The aim of this study was to determine whether Anaplasma phagocytophilum was circulating in olive baboons and vervet monkeys in Laikipia County, Kenya.ResultsSome 146 blood samples collected from olive baboons and 18 from vervet monkeys from Mpala Research Center and Ol jogi Conservancy in Laikipia County were screened for the presence of Anaplasma species using conventional Polymerase Chain Reaction (PCR), and then A. phagocytophilum was confirmed by sequencing using conventional PCR targeting 16S rRNA. This study found an overall prevalence of 18.3% for Anaplasma species. DNA sequences confirmed Anaplasma phagocytophilum in olive baboons for the first time in Kenya.ConclusionThis study provides valuable information on the endemicity of A. phagocytophilum bacteria in olive baboons in Kenya. Future research is needed to establish the prevalence and public health implications of zoonotic A. phagocytophilum isolates and the role of nonhuman primates as reservoirs in the region.
| S-EPMC8669034 | biostudies-literature
Project description:Real-time PCR analysis of a groESL heat shock operon segment showed the presence of two genetic lineages of Anaplasma phagocytophilum in Ixodes ricinus ticks as well as one variant in wild red and roe deer, the latter supposedly representing the natural reservoir of one variant of A. phagocytophilum.
| S-EPMC404603 | biostudies-literature
Project description:THREE INDEPENDENT REPLICATES AND ARE THE CONTROL NON-INFECTED CELLS: GSM49939, GSM49940, GSM49941 THREE INFECTED INDEPEDENDENT REPLICATES: GSM49942, GSM49943, GSM49944 Keywords: ordered
2005-07-29 | GSE2600 | GEO
Project description:Background:Tick-borne diseases are widely distributed among animal populations and are responsible for significant economic losses. However, little attention has been offered for screening such infections world widely. Anaplasma phagocytophilum is among those neglected tick-borne pathogens, particularly in the developing countries. Aim:This study was conducted to detect A. phagocytophilum infection among sheep in three governorates of Iraq (Babylon, Wasit, and Missan) and try to identify the potential tick vector responsible for A. phagocytophilum transmission among sheep in these analyzed regions. Methods:A total of 297 blood samples and 103 ticks were collected and examined for A. phagocytophilum by polymerase chain reaction using specific primers amplifying partial sequence for msp4 gene. Results:The results showed that about 14 out of 297 tested sheep were positive for A. phagocytophilum. There was no difference between A. phygocytophilum prevalence according to animal gender, age, and sampling period. Furthermore, our analysis showed that the main vectors of A. phagocytophilum: Ixodes scapularis, I. pacificus, or I. ricinus were not identified in three regions of Iraq (Rhipicephalus turanicus, Hyalomma anatolicum, and Hyalomma turanicum were identified). Conclusion:These results highlight the importance of the survey of the tick-borne bacterial infections in Iraq and in the Middle East region in general.
| S-EPMC6794391 | biostudies-literature
Project description: Not available
| S-EPMC3320504 | biostudies-literature
Project description:Anaplasma phagocytophilum Genome sequencing
| PRJNA597686 | ENA
Project description:Causative agent for granulocytic anaplasmosis
| PRJNA35205 | ENA