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Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.

ABSTRACT: Phagocytosis is a cellular process for internalization of micron-sized large particles including pathogens. The Bin-Amphiphysin-Rvs167 (BAR) domain proteins, including the FCH-BAR (F-BAR) domain proteins, impose specific morphologies on lipid membranes. Most BAR domain proteins are thought to form membrane invaginations or protrusions by assembling into helical submicron-diameter filaments, such as on clathrin-coated pits, caveolae, and filopodia. However, the mechanism by which BAR domain proteins assemble into micron-scale phagocytic cups was unclear. Here, we show that the two-dimensional sheet-like assembly of Growth Arrest-Specific 7 (GAS7) plays a critical role in phagocytic cup formation in macrophages. GAS7 has the F-BAR domain that possesses unique hydrophilic loops for two-dimensional sheet formation on flat membranes. Super-resolution microscopy reveals the similar assemblies of GAS7 on phagocytic cups and liposomes. The mutations of the loops abolishes both the membrane localization of GAS7 and phagocytosis. Thus, the sheet-like assembly of GAS7 plays a significant role in phagocytosis.

SUBMITTER: Hanawa-Suetsugu K

PROVIDER: S-EPMC6802115 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.

Hanawa-Suetsugu Kyoko K Itoh Yuzuru Y Ab Fatah Maisarah M Nishimura Tamako T Takemura Kazuhiro K Takeshita Kohei K Kubota Satoru S Miyazaki Naoyuki N Wan Mohamad Noor Wan Nurul Izzati WNI Inaba Takehiko T Nguyen Nhung Thi Hong NTH Hamada-Nakahara Sayaka S Oono-Yakura Kayoko K Tachikawa Masashi M Iwasaki Kenji K Kohda Daisuke D Yamamoto Masaki M Kitao Akio A Shimada Atsushi A Suetsugu Shiro S

Nature communications 20191018 1

Phagocytosis is a cellular process for internalization of micron-sized large particles including pathogens. The Bin-Amphiphysin-Rvs167 (BAR) domain proteins, including the FCH-BAR (F-BAR) domain proteins, impose specific morphologies on lipid membranes. Most BAR domain proteins are thought to form membrane invaginations or protrusions by assembling into helical submicron-diameter filaments, such as on clathrin-coated pits, caveolae, and filopodia. However, the mechanism by which BAR domain prote ...[more]

PMID: 31628328

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Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.

Publications

Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7.

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