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Effect of caloric restriction and rapamycin on ovarian aging in mice.

ABSTRACT: Caloric restriction (CR) increases the preservation of the ovarian primordial follicular reserve, which can potentially delay menopause. Rapamycin also increases preservation on the ovarian reserve, with similar mechanism to CR. Therefore, the aim of our study was to evaluate the effects of rapamycin and CR on metabolism, ovarian reserve, and gene expression in mice. Thirty-six female mice were allocated into three groups: control, rapamycin-treated (4 mg/kg body weight every other day), and 30% CR. Caloric restricted females had lower body weight (P??0.05) and induced insulin resistance (P?

SUBMITTER: Garcia DN

PROVIDER: S-EPMC6815295 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Effect of caloric restriction and rapamycin on ovarian aging in mice.

Garcia Driele N DN Saccon Tatiana D TD Pradiee Jorgea J Rincón Joao A A JAA Andrade Kelvin R S KRS Rovani Monique T MT Mondadori Rafael G RG Cruz Luis A X LAX Barros Carlos C CC Masternak Michal M MM Bartke Andrzej A Mason Jeffrey B JB Schneider Augusto A

GeroScience 20190729 4

Caloric restriction (CR) increases the preservation of the ovarian primordial follicular reserve, which can potentially delay menopause. Rapamycin also increases preservation on the ovarian reserve, with similar mechanism to CR. Therefore, the aim of our study was to evaluate the effects of rapamycin and CR on metabolism, ovarian reserve, and gene expression in mice. Thirty-six female mice were allocated into three groups: control, rapamycin-treated (4 mg/kg body weight every other day), and 30% ...[more]

PMID: 31359237

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Altered proteome turnover and remodeling by short-term caloric restriction or rapamycin rejuvenate the aging heart.

Project description:Chronic caloric restriction (CR) and rapamycin inhibit the mechanistic target of rapamycin (mTOR) signaling, thereby regulating metabolism and suppressing protein synthesis. Caloric restriction or rapamycin extends murine lifespan and ameliorates many aging-associated disorders; however, the beneficial effects of shorter treatment on cardiac aging are not as well understood. Using a recently developed deuterated-leucine labeling method, we investigated the effect of short-term (10 weeks) CR or rapamycin on the proteomics turnover and remodeling of the aging mouse heart. Functionally, we observed that short-term CR and rapamycin both reversed the pre-existing age-dependent cardiac hypertrophy and diastolic dysfunction. There was no significant change in the cardiac global proteome (823 proteins) turnover with age, with a median half-life 9.1 days in the 5-month-old hearts and 8.8 days in the 27-month-old hearts. However, proteome half-lives of old hearts significantly increased after short-term CR (30%) or rapamycin (12%). This was accompanied by attenuation of age-dependent protein oxidative damage and ubiquitination. Quantitative proteomics and pathway analysis revealed an age-dependent decreased abundance of proteins involved in mitochondrial function, electron transport chain, citric acid cycle, and fatty acid metabolism as well as increased abundance of proteins involved in glycolysis and oxidative stress response. This age-dependent cardiac proteome remodeling was significantly reversed by short-term CR or rapamycin, demonstrating a concordance with the beneficial effect on cardiac physiology. The metabolic shift induced by rapamycin was confirmed by metabolomic analysis.

| S-EPMC4040127 | biostudies-literature

Effect of Caloric Restriction on the in vivo Functional Properties of Aging Microglia.

Project description:Throughout the lifespan, microglia, the primary innate immune cells of the brain, fulfill a plethora of homeostatic as well as active immune defense functions, and their aging-induced dysfunctionality is now considered as a key trigger of aging-related brain disorders. Recent evidence suggests that both organism's sex and age critically impact the functional state of microglia but in vivo determinants of such state(s) remain unclear. Therefore, we analyzed in vivo the sex-specific functional states of microglia in young adult, middle aged and old wild type mice by means of multicolor two-photon imaging, using the microglial Ca2 + signaling and directed process motility as main readouts. Our data revealed the sex-specific differences in microglial Ca2 + signaling at all ages tested, beginning with young adults. Furthermore, for both sexes it showed that during the lifespan the functional state of microglia changes at least twice. Already at middle age the cells are found in the reactive or immune alerted state, characterized by heightened Ca2 + signaling but normal process motility whereas old mice harbor senescent microglia with decreased Ca2 + signaling, and faster but disorganized directed movement of microglial processes. The 6-12 months long caloric restriction (70% of ad libitum food intake) counteracted these aging-induced changes shifting many but not all functional properties of microglia toward a younger phenotype. The improvement of Ca2 + signaling was more pronounced in males. Importantly, even short-term (6-week-long) caloric restriction beginning at old age strongly improved microglial process motility and induced a significant albeit weaker improvement of microglial Ca2 + signaling. Together, these data provide first sex-specific in vivo characterization of functional properties of microglia along the lifespan and identify caloric restriction as a potent, cost-effective, and clinically relevant tool for rejuvenation of microglia.

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Expression data from yeasts under non-caloric restriction (NR), caloric restriction (CR) and rapamycin treatment (RM)

Project description:We performed microarrays to identify change of gene expression under NR, CR, and RM and found differentially expressed genes between each condition.

2017-06-30 | GSE82324 | GEO

Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice.

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| S-EPMC58517 | biostudies-literature

A low dose of dietary resveratrol partially mimics caloric restriction and retards aging parameters in mice.

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Epigenetic aging signatures in mice livers are slowed by dwarfism, calorie restriction and rapamycin treatment.

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caloric restriction

Project description:caloric restriction

| PRJNA87197 | ENA

Caloric restriction enhances fear extinction learning in mice.

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