Ontology highlight
ABSTRACT:
SUBMITTER: Shigematsu M
PROVIDER: S-EPMC6853296 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Shigematsu Megumi M Morichika Keisuke K Kawamura Takuya T Honda Shozo S Kirino Yohei Y
PLoS genetics 20191113 11
RNA molecules generated by ribonuclease cleavage sometimes harbor a 2',3'-cyclic phosphate (cP) at their 3'-ends. Those cP-containing RNAs (cP-RNAs) form a hidden layer of transcriptome because standard RNA-seq cannot capture them as a result of cP's prevention of an adapter ligation reaction. Here we provide genome-wide analyses of short cP-RNA transcriptome across multiple mouse tissues. Using cP-RNA-seq that can exclusively sequence cP-RNAs, we identified numerous novel cP-RNA species which a ...[more]