Dataset Information
A high-throughput screening identifies histone deacetylase inhibitors as therapeutic agents against medulloblastoma.
Ontology highlight
ABSTRACT: Background
Medulloblastoma is the most frequently occurring malignant brain tumor in children. Current treatment strategies for medulloblastoma include aggressive surgery, cranio-spinal irradiation and adjuvant chemotherapy. Because current treatments can cause severe long-term side effects and are not curative, successful treatment remains a challenge.Methods
In this study, we employed a high-throughput cell viability assay to screen 12,800 compounds and to identify drug candidates with anti-proliferative properties for medulloblastoma cells. We also tested these compounds for attenuating medulloblastoma tumor development using mouse xenografts.Results
We identified two histone deacetylase inhibitors (dacinostat and quisinostat) with anti-proliferative properties for medulloblastoma cells. We showed that both compounds induce cytotoxicity, trigger cell apoptosis, and block cell cycle progression at the G2/M phase. In addition, dacinostat and quisinostat attenuated xenograft medulloblastoma growth in mice.Conclusions
Our findings suggest that histone deacetylase inhibitors are potent therapeutic agents against medulloblastoma.
SUBMITTER: Zhang S
PROVIDER: S-EPMC6858705 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
A high-throughput screening identifies histone deacetylase inhibitors as therapeutic agents against medulloblastoma.
Experimental hematology & oncology 20191115
<h4>Background</h4>Medulloblastoma is the most frequently occurring malignant brain tumor in children. Current treatment strategies for medulloblastoma include aggressive surgery, cranio-spinal irradiation and adjuvant chemotherapy. Because current treatments can cause severe long-term side effects and are not curative, successful treatment remains a challenge.<h4>Methods</h4>In this study, we employed a high-throughput cell viability assay to screen 12,800 compounds and to identify drug candida ...[more]