Dataset Information

Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.

ABSTRACT: Transforming growth factor beta 3 (TGF?3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGF?3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGF?3 in an in vitro model. TGF?3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGF?3 were not detected in the rinsing fluid of the TGF?3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) were then seeded on scaffolds loaded with 300 ng TGF?3 to assess its bioactivity. Both scaffold-associated TGF?3 and TGF?3 dissolved in the cell culture medium, the latter serving as control group, promoted elongation of cell shapes and scaffold contraction (p < 0.05). Furthermore, scaffold-associated and dissolved TGF?3 affected MSC musculoskeletal gene expression in a similar manner, with an upregulation of tenascin c and downregulation of other matrix molecules, most markedly decorin (p < 0.05). These results demonstrate that the bioactivity of scaffold-associated TGF?3 is preserved, thus TGF?3 application via absorption in decellularized tendon scaffolds is a feasible approach.

SUBMITTER: Roth SP

PROVIDER: S-EPMC6862173 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.

Roth Susanne Pauline SP Brehm Walter W Groß Claudia C Scheibe Patrick P Schubert Susanna S Burk Janina J

International journal of molecular sciences 20191103 21

Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. TGFβ3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGFβ3 were not detected in the rinsing fluid of the TGFβ3-loaded scaffolds. Equine adipos ...[more]

PMID: 31684150

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Dataset Information

Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.

Publications

Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering.

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