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Analysis of tRNACys processing in the absence of CCAase in Bacillus subtilis.


ABSTRACT: In Bacillus subtilis, the tRNACys lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNACys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNACys.

SUBMITTER: Campos Guillen J 

PROVIDER: S-EPMC6863329 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Analysis of tRNA<sup>Cys</sup> processing in the absence of CCAase in Bacillus subtilis.

Campos Guillén Juan J   Arvizu Gómez Jackeline Lizzeta JL   Jones George H GH   Hernández Flores José Luis JL   Ramos López Miguel Angel MA   Cruz Hernández Andrés A   Romero Gómez Sergio S  

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20190412 3


In Bacillus subtilis, the tRNA<sup>Cys</sup> lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNA<sup>Cys</sup> processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNA<sup>Cys</sup>. ...[more]

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2015-01-26 | GSE65272 | GEO