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Complete inclusion of bioactive molecules and particles in polydimethylsiloxane: a straightforward process under mild conditions.

ABSTRACT: By applying a slow curing process, we show that biomolecules can be incorporated via a simple process as liquid stable phases inside a polydimethylsiloxane (PDMS) matrix. The process is carried out under mild conditions with regards to temperature, pH and relative humidity, and is thus suitable for application to biological entities. Fluorescence and enzymatic activity measurements show that the biochemical properties of the proteins and enzyme tested are preserved, without loss due to adsorption at the liquid-polymer interface. Protected from external stimuli by the PDMS matrix, these soft liquid composite materials are new tools of interest for robotics, microfluidics, diagnostics and chemical microreactors.

SUBMITTER: Faccio G

PROVIDER: S-EPMC6879495 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Complete inclusion of bioactive molecules and particles in polydimethylsiloxane: a straightforward process under mild conditions.

Faccio Greta G Cont Alice A Mailand Erik E Zare-Eelanjegh Elaheh E Innocenti Malini Riccardo R Maniura-Weber Katharina K Rossi René M RM Spano Fabrizio F

Scientific reports 20191126 1

By applying a slow curing process, we show that biomolecules can be incorporated via a simple process as liquid stable phases inside a polydimethylsiloxane (PDMS) matrix. The process is carried out under mild conditions with regards to temperature, pH and relative humidity, and is thus suitable for application to biological entities. Fluorescence and enzymatic activity measurements show that the biochemical properties of the proteins and enzyme tested are preserved, without loss due to adsorptio ...[more]

PMID: 31772250

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