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Controlled Growth of Dendrimer-Coated Gold Nanoparticles: A Solvent-Free Process in Mild Conditions.

ABSTRACT: Monodisperse dendrimer-coated gold nanoparticles with a spherical shape have been obtained by direct reduction of HAuCl₄ with sodium borohydride in the presence of dodecanethiol as a stabilizer and subsequent functionalization by ligand exchange reaction with polybenzylic thiolated dendrons. The substitution pattern of the dendrimeric units plays a fundamental role in the rate of the functionalization exchange process and consequently conditions the size and the polydispersity of the NPs obtained. An ulterior growth process occurs by thermal stimuli (150 °C) in a solvent-free environment. This method, carried out in mild conditions, allows the formation of highly monodisperse gold NPs with different sizes for different time reactions, and we discuss the mechanisms involved in this process. Finally, we demonstrate the chemical composition and stability of our compounds by structural, thermal, and chemical characterization of the samples before and after thermal treatment.

SUBMITTER: Ulloa JA

PROVIDER: S-EPMC7807749 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Controlled Growth of Dendrimer-Coated Gold Nanoparticles: A Solvent-Free Process in Mild Conditions.

Ulloa José Antonio JA Barberá Joaquín J Serrano José Luis JL

ACS omega 20201222 1

Monodisperse dendrimer-coated gold nanoparticles with a spherical shape have been obtained by direct reduction of HAuCl<sub>4</sub> with sodium borohydride in the presence of dodecanethiol as a stabilizer and subsequent functionalization by ligand exchange reaction with polybenzylic thiolated dendrons. The substitution pattern of the dendrimeric units plays a fundamental role in the rate of the functionalization exchange process and consequently conditions the size and the polydispersity of the ...[more]

PMID: 33458486

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Project description:BackgroundEngineered inorganic nanoparticles (NPs) are essential components in the development of nanotechnologies. For applications in nanomedicine, particles need to be functionalized to ensure a good dispersibility in biological fluids. In many cases however, functionalization is not sufficient: the particles become either coated by a corona of serum proteins or precipitate out of the solvent. We show that by changing the coating of magnetic iron oxide NPs using poly-L-lysine (PLL) polymer the colloidal stability of the dispersion is improved in aqueous solutions including water, phosphate buffered saline (PBS), PBS with 10% fetal bovine serum (FBS) and cell culture medium, and the internalization of the NPs toward living mammalian cells is profoundly affected.MethodsA multifunctional magnetic NP is designed to perform a near-infrared (NIR)-responsive remote control photothermal ablation for the treatment of breast cancer. In contrast to the previously reported studies of gold (Au) magnetic (Fe3O4) core-shell NPs, a Janus-like nanostructure is synthesized with Fe3O4 NPs decorated with Au resulting in an approximate size of 60 nm mean diameter. The surface of trisoctahedral Au-Fe3O4 NPs was coated with a positively charged polymer, PLL to deliver the NPs inside cells. The PLL-Au-Fe3O4 NPs were characterized by transmission electron microscopy (TEM), XRD, FT-IR and dynamic light scattering (DLS). The unique properties of both Au surface plasmon resonance and superparamagnetic moment result in a multimodal platform for use as a nanothermal ablator and also as a magnetic resonance imaging (MRI) contrast agent, respectively. Taking advantage of the photothermal therapy, PLL-Au-Fe3O4 NPs were incubated with BT-474 and MDA-MB-231 breast cancer cells, investigated for the cytotoxicity and intracellular uptake, and remotely triggered by a NIR laser of ~ 808 nm (1 W/cm2 for 10 min).ResultsThe PLL coating increased the colloidal stability and robustness of Au-Fe3O4 NPs (PLL-Au-Fe3O4) in biological media including cell culture medium, PBS and PBS with 10% fetal bovine serum. It is revealed that no significant (< 10%) cytotoxicity was induced by PLL-Au-Fe3O4 NPs itself in BT-474 and MDA-MB-231 cells at concentrations up to 100 μg/ml. Brightfield microscopy, fluorescence microscopy and TEM showed significant uptake of PLL-Au-Fe3O4 NPs by BT-474 and MDA-MB-231 cells. The cells exhibited 40 and 60% inhibition in BT-474 and MDA-MB-231 cell growth, respectively following the internalized NPs were triggered by a photothermal laser using 100 μg/ml PLL-Au-Fe3O4 NPs. The control cells treated with NPs but without laser showed < 10% cell death compared to no laser treatment control CONCLUSION: Combined together, the results demonstrate a new polymer gold superparamagnetic nanostructure that integrates both diagnostics function and photothermal ablation of tumors into a single multimodal nanoplatform exhibiting a significant cancer cell death.

Dataset Information

Controlled Growth of Dendrimer-Coated Gold Nanoparticles: A Solvent-Free Process in Mild Conditions.

Publications

Controlled Growth of Dendrimer-Coated Gold Nanoparticles: A Solvent-Free Process in Mild Conditions.

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