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Downregulation of microRNA-30a-5p contributes to the replication of duck enteritis virus by regulating Beclin-1-mediated autophagy.


ABSTRACT: BACKGROUND:MicroRNAs (miRNAs) is increasingly recognized as an important element in regulating virus-host interactions. Our previous results showed that cellular miR-30a-5p was significantly downregulated after duck enteritis virus (DEV) infection cell. However, whehter or not the miR-30a-5p is involved in DEV infection has not been known. METHODS:Quantitative reverse-transcription PCR (qRT-PCR) was used to measure the expression levels of miRNAs(miR-30a-5p) and Beclin-1 mRNA. The miR-30a-5p - Beclin-1 target interactions were determined by Dual luciferase reporter assay (DLRA). Western blotting was utilized to analyze Beclin-1-mediated duck embryo fibroblast (DEF) cells autophagy activity. DEV titers were estimated by the median tissue culture infective dose (TCID50). RESULTS:The miR-30a-5p was significantly downregulated and the Beclin-1 mRNA was significantly upregulated in DEV-infected DEF cells. DLRA confirmed that miR-30a-5p directly targeted the 3'- UTR of the Beclin-1 gene. Overexpression of miR-30a-5p significantly reduced the expression level of Beclin-1protein (p?

SUBMITTER: Wu X 

PROVIDER: S-EPMC6880601 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Downregulation of microRNA-30a-5p contributes to the replication of duck enteritis virus by regulating Beclin-1-mediated autophagy.

Wu Xianglong X   Jia Renyong R   Wang Mingshu M   Chen Shun S   Liu Mafeng M   Zhu Dekang D   Zhao Xinxin X   Yang Qiao Q   Wu Ying Y   Yin Zhongqiong Z   Zhang Shaqiu S   Huang Juan J   Zhang Ling L   Liu Yunya Y   Yu Yanling Y   Pan Leichang L   Tian Bin B   Rehman Mujeeb Ur MU   Chen Xiaoyue X   Cheng Anchun A  

Virology journal 20191126 1


<h4>Background</h4>MicroRNAs (miRNAs) is increasingly recognized as an important element in regulating virus-host interactions. Our previous results showed that cellular miR-30a-5p was significantly downregulated after duck enteritis virus (DEV) infection cell. However, whehter or not the miR-30a-5p is involved in DEV infection has not been known.<h4>Methods</h4>Quantitative reverse-transcription PCR (qRT-PCR) was used to measure the expression levels of miRNAs(miR-30a-5p) and Beclin-1 mRNA. The  ...[more]

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2020-06-27 | GSE100524 | GEO