Improved Electrophoretic Separation to Assist the Monitoring of Bcl-xL Post-Translational Modifications.
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ABSTRACT: Bcl-xL is an oncogene of which the survival functions are finely tuned by post-translational modifications (PTM). Within the Bcl-2 family of proteins, Bcl-xL shows unique eligibility to deamidation, a time-related spontaneous reaction. Deamidation is still a largely overlooked PTM due to a lack of easy techniques to monitor Asn?Asp/IsoAsp conversions or Glu?Gln conversions. Being able to detect PTMs is essential to achieve a comprehensive description of all the regulatory mechanisms and functions a protein can carry out. Here, we report a gel composition improving the electrophoretic separation of deamidated forms of Bcl-xL generated either by mutagenesis or by alkaline treatment. Importantly, this new gel formulation proved efficient to provide the long-sought evidence that even doubly-deamidated Bcl-xL remains eligible for regulation by phosphorylation.
SUBMITTER: Bobo C
PROVIDER: S-EPMC6888115 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
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