Unknown

Dataset Information

0

Par-1 controls the composition and growth of cortical actin caps during Drosophila embryo cleavage.


ABSTRACT: Cell structure depends on the cortex, a thin network of actin polymers and additional proteins underlying the plasma membrane. The cell polarity kinase Par-1 is required for cells to form following syncytial Drosophila embryo development. This requirement stems from Par-1 promoting cortical actin caps that grow into dome-like metaphase compartments for dividing syncytial nuclei. We find the actin caps to be a composite material of Diaphanous (Dia)-based actin bundles interspersed with independently formed, Arp2/3-based actin puncta. Par-1 and Dia colocalize along extended regions of the bundles, and both are required for the bundles and for each other's bundle-like localization, consistent with an actin-dependent self-reinforcement mechanism. Par-1 helps establish or maintain these bundles in a cortical domain with relatively low levels of the canonical formin activator Rho1-GTP. Arp2/3 is required for displacing the bundles away from each other and toward the cap circumference, suggesting interactions between these cytoskeletal components could contribute to the growth of the cap into a metaphase compartment.

SUBMITTER: Jiang T 

PROVIDER: S-EPMC6891076 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Par-1 controls the composition and growth of cortical actin caps during <i>Drosophila</i> embryo cleavage.

Jiang Tao T   Harris Tony J C TJC  

The Journal of cell biology 20191022 12


Cell structure depends on the cortex, a thin network of actin polymers and additional proteins underlying the plasma membrane. The cell polarity kinase Par-1 is required for cells to form following syncytial <i>Drosophila</i> embryo development. This requirement stems from Par-1 promoting cortical actin caps that grow into dome-like metaphase compartments for dividing syncytial nuclei. We find the actin caps to be a composite material of Diaphanous (Dia)-based actin bundles interspersed with ind  ...[more]

Similar Datasets

| S-EPMC4084522 | biostudies-literature
| S-EPMC3177071 | biostudies-literature
| S-EPMC2848566 | biostudies-literature
| S-EPMC2171764 | biostudies-literature
| S-EPMC3216648 | biostudies-literature
| S-EPMC5100295 | biostudies-literature
| S-EPMC10398877 | biostudies-literature
| S-EPMC3204067 | biostudies-literature
| S-EPMC4197085 | biostudies-literature
| S-EPMC3818562 | biostudies-literature