Ontology highlight
ABSTRACT:
SUBMITTER: Xu S
PROVIDER: S-EPMC6895266 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Xu Shan S Ci Yali Y Wang Leijie L Yang Yang Y Zhang Leiliang L Xu Caimin C Qin Chengfeng C Shi Lei L
Nucleic acids research 20190901 16
Zika virus is a positive single-strand RNA virus whose replication involved RNA unwinding and synthesis. ZIKV NS3 contains a helicase domain, but its enzymatic activity is not fully characterized. Here, we established a dsRNA unwinding assay based on the FRET effect to study the helicase activity of ZIKV NS3, which provided kinetic information in real time. We found that ZIKV NS3 specifically unwound dsRNA/dsDNA with a 3' overhang in the 3' to 5' direction. The RNA unwinding ability of NS3 signi ...[more]