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Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

ABSTRACT: Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.

SUBMITTER: Clerici M

PROVIDER: S-EPMC6900284 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.

Clerici Marcello M Faini Marco M Muckenfuss Lena M LM Aebersold Ruedi R Jinek Martin M

Nature structural & molecular biology 20180122 2

Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mamma ...[more]

PMID: 29358758

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