Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex

ABSTRACT: Structural study of the polyA signal recognition by the human CPSF using X-ray crystallography, cross-linking MS, pull-downs and fluorescence polarisation assays.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Marco Faini

LAB HEAD: Ruedi Aebersold

PROVIDER: PXD008122 | Pride | 2017-12-18

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Figure_1-Source_Data_1.xlsx	Xlsx
	mfaini_C1506_106.c.mzXML	Mzxml
	mfaini_C1506_107.c.mzXML	Mzxml
	mfaini_C1506_109.c.mzXML	Mzxml
	mfaini_C1506_110.c.mzXML	Mzxml

Items per page:

1 - 5 of 5

Publications

Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex.

Clerici Marcello M Faini Marco M Aebersold Ruedi R Jinek Martin M

eLife 20171223

3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is dependent on the recognition of the hexanucleotide AAUAAA motif in the pre-mRNA polyadenylation signal by the cleavage and polyadenylation specificity factor (CPSF) complex. A core CPSF complex comprising CPSF160, WDR33, CPSF30 and Fip1 is sufficient for AAUAAA motif recognition, yet the molecular interactions underpinning its assembly and mechanism of PAS recognition are not understood. Based on ...[more]

PMID: 29274231

Similar Datasets

Cryo-EM structure of the full human PRC2 and regulation by AEBP2 and JARID2

Project description:Structural study of the PRC2 complex and its regulators AEPB2 and JARID2 combining cryo-EM, cross-linking, targeted MS and hydrogen/deuterium exchange MS.

2018-01-25 | PXD008605 | Pride

pH dependence of succinimide ester-based protein cross-linking for structural mass spectrometry applications

Project description:The pH dependency of the reaction of the cross-linking reagent, disuccinimidyl suberate (DSS), was studied on a set of eight model proteins.

2021-11-15 | PXD027891 | Pride

The human GID complex engages two independent modules for substrate recruitment

Project description:Chemical cross-linking coupled to mass spectrometry was used to study two different assemblies of the human E3 ubiquitin ligase complex, GID. The 5mer complex consisted of subunits MAEA, RMND5a, TWA1, WDR26, and RanBP9; the 6mer complex consisted of the subunits of the 5mer complex and the additional subunit ARMC8β. Cross-linking experiments were performed with disuccinimidyl suberate (DSS) or pimelic dihydrazide (PDH) in combination with the coupling reagent DMTMM.

2021-08-25 | PXD024822 | Pride

Structural requirements for photo-induced RNA-protein cross-linking

Project description:Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the UV cross-linking behavior of in vitro reconstituted FOX1 RRM in complex with its cognate RNA sequence, the Fox binding element (FBE), (U)GCAUGU. The FBE heptanucleotide was subsequently mutated, and the impact on UV cross-linking and affinity investigated. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.

2022-06-09 | PXD031381 | Pride

Sequence specific recognition of U1 snRNP stem-loop 4 by the ubiquitin-like domain of U2 snRNP protein SF3A1 and its RGG motif

Project description:Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the ubiquitin-like domain (UBL) of the protein SF3A1, a component of the U2 snRNP, with stem-loop 4 RNA (SL4) from the U1 snRNP. The complex was cross-linked and analysed both in isolation, and in broader context with components of the U1 snRNP. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.

2022-02-22 | PXD027189 | Pride

Sensitive detection and structural characterisation of UV-induced cross-links in protein-ribonucleic acid complexes using stable isotope labelling and mass spectrometry

Project description:The recently introduced cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) technique enables protein-RNA cross-links to be used as precisely localized distance restraints in de novo structural modelling, but little is known about the structural characteristics of UV-induced cross-links. Here we demonstrate protocol optimizations, and apply the enhanced protocol to a set of model protein-RNA complexes to better characterize the properties of UV-induced protein-RNA cross-links. We use these insights to study a non-canonical protein-RNA interaction between SF3A1 of the U2 snRNP and stem-loop 4 of the U1 snRNP, demonstrating that UV cross-linking and mass spectrometry is a reliable standalone data type for low resolution structural characterizations of protein-RNA interactions.

2023-09-06 | PXD029930 | Pride

Molecular architecture of the human tRNA ligase complex

Project description:Chemical cross-linking coupled to mass spectrometry was used to study two tRNA ligase complexes, one consisting of subunits RTCB, DDX1, CGI-99, FAM98B and Ashwin (“full tRLC”) and one lacking Ashwin (“core tRLC”). Cross-linking was performed using the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2021-12-15 | PXD025662 | Pride

CASP XL-MS target T0985: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)

Project description:Chemical cross-linking coupled to mass spectrometry was used to provide distance restraints for different homo- and heterooligomeric complexes as part of the CASP13 experiment (https://www.predictioncenter.org/casp13/index.cgi).

2019-10-02 | PXD010483 | Pride

CASP XL-MS target T0981: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)

2019-10-02 | PXD010384 | Pride

CASP XL-MS target T0987: Cross-linking for the 13th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP13)

2019-10-02 | PXD010410 | Pride