Unknown

Dataset Information

0

Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway.


ABSTRACT: A number of antimalarial drugs interfere with the electron transport chain and heme-related reactions; however, the biosynthesis of heme derivatives in Plasmodium parasites has not been fully elucidated. Here, we characterized the steps that lead to the farnesylation of heme. After the identification of a gene encoding heme O synthase, we identified heme O synthesis in blood stage parasites through the incorporation of radioactive precursors. The presence of heme O synthesis in intraerythrocytic stages of Plasmodium falciparum was confirmed by mass spectrometry. Inabenfide and uniconazole-P appeared to interfere in heme synthesis, accordingly, parasite growth was also affected by the addition of these drugs. We conclude that heme O synthesis occurs in blood stage-P. falciparum and this pathway could be a potential target for antimalarial drugs.

SUBMITTER: Simao-Gurge RM 

PROVIDER: S-EPMC6917786 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum and potential inhibitors of this pathway.

Simão-Gurge Raquel M RM   Wunderlich Gerhard G   Cricco Julia A JA   Cubillos Eliana F Galindo EFG   Doménech-Carbó Antonio A   Cebrián-Torrejón Gerardo G   Almeida Fernando G FG   Cirulli Brenda A BA   Katzin Alejandro M AM  

Scientific reports 20191217 1


A number of antimalarial drugs interfere with the electron transport chain and heme-related reactions; however, the biosynthesis of heme derivatives in Plasmodium parasites has not been fully elucidated. Here, we characterized the steps that lead to the farnesylation of heme. After the identification of a gene encoding heme O synthase, we identified heme O synthesis in blood stage parasites through the incorporation of radioactive precursors. The presence of heme O synthesis in intraerythrocytic  ...[more]

Similar Datasets

| S-EPMC2665121 | biostudies-literature
| S-EPMC4784480 | biostudies-literature
| S-EPMC3049507 | biostudies-other
| S-EPMC3059058 | biostudies-literature
| S-EPMC5896089 | biostudies-other
| S-EPMC5292867 | biostudies-literature
| S-EPMC7582821 | biostudies-literature
| S-EPMC3601789 | biostudies-literature
| S-EPMC6170468 | biostudies-literature
| S-EPMC2726426 | biostudies-other