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Assessment of Phenotype Microarray plates for rapid and high-throughput analysis of collateral sensitivity networks.

ABSTRACT: The crisis of antimicrobial resistance is driving research into the phenomenon of collateral sensitivity. Sometimes, when a bacterium evolves resistance to one antimicrobial, it becomes sensitive to others. In this study, we have investigated the utility of Phenotype Microarray (PM) plates for identifying collateral sensitivities with unprecedented throughput. We assessed the relative resistance/sensitivity phenotypes of nine strains of Staphylococcus aureus (two laboratory strains and seven clinical isolates) towards the 72 antimicrobials contained in three PM plates. In general, the PM plates reported on resistance and sensitivity with a high degree of reproducibility. However, a rigorous comparison of PM growth phenotypes with minimum inhibitory concentration (MIC) measurements revealed a trade-off between throughput and accuracy. Small differences in PM growth phenotype did not necessarily correlate with changes in MIC. Thus, we conclude that PM plates are useful for the rapid and high-throughput assessment of large changes in collateral sensitivity phenotypes during the evolution of antimicrobial resistance, but more subtle examples of cross-resistance or collateral sensitivity cannot be identified reliably using this approach.

SUBMITTER: Dunkley EJ

PROVIDER: S-EPMC6919586 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Assessment of Phenotype Microarray plates for rapid and high-throughput analysis of collateral sensitivity networks.

Dunkley Elsie J EJ Chalmers James D JD Cho Stephanie S Finn Thomas J TJ Patrick Wayne M WM

PloS one 20191218 12

The crisis of antimicrobial resistance is driving research into the phenomenon of collateral sensitivity. Sometimes, when a bacterium evolves resistance to one antimicrobial, it becomes sensitive to others. In this study, we have investigated the utility of Phenotype Microarray (PM) plates for identifying collateral sensitivities with unprecedented throughput. We assessed the relative resistance/sensitivity phenotypes of nine strains of Staphylococcus aureus (two laboratory strains and seven cli ...[more]

PMID: 31851668

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Assessment of Phenotype Microarray plates for rapid and high-throughput analysis of collateral sensitivity networks.

Publications

Assessment of Phenotype Microarray plates for rapid and high-throughput analysis of collateral sensitivity networks.

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